Purification and Characterization of A New Fibrinogenase From The Venom of the Chinese Habu Snake (Trimeresurus mucrosquamatus)
文献类型：期刊论文
| 作者 | Zhang Y; Xiong YL |
| 刊名 | 动物学研究
 |
| 出版日期 | 1991 |
| 卷号 | 12期号:2页码:199-207 |
| 关键词 | Snake venom Fibrinogenase Proteinase |
| ISSN号 | 0254-5853 |
| 其他题名 | 烙铁头蛇毒纤维蛋白原溶酶研究 Ⅰ分离纯化及理化性质 |
| 中文摘要 | 通过DEAE-SephadexA-50,DEAE-SepharoseCL-6B,MonoQ（FPLC）三步离子交换柱层析,纯化得到一新的纤维蛋白原溶酶,在碱性聚丙烯酰胺凝胶电泳和SDS-聚丙烯酰胺凝胶电泳上均呈单一的蛋白带。分子量为26000,等电点pI4.7;它是一个糖蛋白,含糖量6.4％,其中中性糖0.3％,己糖胺4.9％,唾液酸1.2％。烙铁头蛇毒纤维蛋白原溶酶TMVFg由187个氨基酸组成,含有较高的酸性氨基酸,此外甘氨酸含量也较高。TMVFg热稳定性强,而酸不稳定,在280nm处具有典型的蛋白吸收峰,在无离子水中紫外消光系数E0.1％/280nm=1.558。纯化的TMVFS具有较强的精氨酸酯酶活力,对苯甲酰-L-精氨酸乙酯（BAEE）的Km值为1.4×10-3M。TMVFS的活性受苯甲基丹磺酰氟（PMSF）抑制,乙二胺四乙酸（EDTA）对活性无影响。TMVFg不能使纤维蛋白原凝固,但能水解纤维蛋白原α、β链。纤溶实验表明TMVFg具有激活纤溶作用。纯化的烙铁头蛇毒纤维蛋白原溶酶对酪蛋白无作用,无出血活性,因而与凝血酶样酶、出血毒素及纤维蛋白原溶酶（OUYANG et al,1977） 明显不同。 |
| 英文摘要 | A new fibrinogenase (EC 3.4.21.5) was isolated and purified from the venom of Chinese habu snake (Trimeresurus mucrosquamatus) by DEAE-SephadexA-50,DEAE-Sepharose CL-6B,MonoQ (FPLC) cclumn chromatography.It showed a single protein band both in sodium dodecyl sulfate (SDS)-polyacrylamide gel egectrophoresis and alkaline polyacrylamide gel electrophoresis.The molecular weight was estimated to be 26000 by SDS-polyacrylamide gel electrophoresis.The isoelectric point was found to be Ph 4.7.It was a glycoprotein containing 6.4% carbohydrate with 0.3% neutral sugar,1.2% sialic acid,4.9% hexosamine.It was composed of about 178 amino acid residues and rich in glycine and aspartic acid.The fibrinogenase of the venom of T.mucrosquamatus TWVFg was heat stable but labile to acid.Its extinction coefficient (1 mg/ml) at 280 nm was 1.558.Purified TMVFg had strong arginine esterase activity;the Km to benzoylarginiue ethylester (BAEE) was 1.4×10[-3]M.The enzyme activity could be inhibited by pheny.Imethanesulfonyfluoride (PMSF);but was not affected by ethylenediamine tetraacetic acid (EDTA).TMVFg had fibrinogenolytic activity;electrophoresis of fibrinogen degraded with TMVFg revealed the rapid disappearance of the α (alpha) and β (beta)-chains and the appearance of lower molecular weight frag ments.TMVFg did not cause fibrinogen solution clotting,nor coagulating plasma and showed neither hemorrhagic activity nor proteolytic activity toward casein.TMVFg had activating fibrinolytic activity. |
| 收录类别 | 其他 |
| 原文出处 | 19911202199.pdf |
| 语种 | 中文 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/1977]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_其他 |
| 作者单位 | Kunming Institute of Zoology,Academia Sinica |
| 推荐引用方式 GB/T 7714 | Zhang Y,Xiong YL. Purification and Characterization of A New Fibrinogenase From The Venom of the Chinese Habu Snake (Trimeresurus mucrosquamatus)[J]. 动物学研究,1991,12(2):199-207. |
| APA | Zhang Y,&Xiong YL.(1991).Purification and Characterization of A New Fibrinogenase From The Venom of the Chinese Habu Snake (Trimeresurus mucrosquamatus).动物学研究,12(2),199-207. |
| MLA | Zhang Y,et al."Purification and Characterization of A New Fibrinogenase From The Venom of the Chinese Habu Snake (Trimeresurus mucrosquamatus)".动物学研究 12.2(1991):199-207. |
入库方式： OAI收割
来源：昆明动物研究所
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