Molecular cloning and characterization of a platelet glycoprotein Ib-binding protein from the venom of Trimeresurus stejnegeri
文献类型：期刊论文
| 作者 | Lee WH1,2; Zhang Y[*]1 |
| 刊名 | TOXICON
 |
| 出版日期 | 2003 |
| 卷号 | 41期号:7页码:885-892 |
| 关键词 | Snake venom Glycoprotein Ib-binding protein Trimeresurus stejnegeri cDNA sequence |
| ISSN号 | 0041-0101 |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | A platelet glycoprotein Ib-binding protein, termed TSV-GPIb-BP, was isolated from the venom of Trimeresurus stejnegeri. On SDS-polyacrylamide gel electrophoresis, TSV-GPIb-BP showed a single band with an apparent molecular weight of 28,000 and two distinct bands with apparent molecular weights of 16,000 and 15,000 under non-reducing and reducing conditions, respectively. cDNA clones containing the coding sequences for both TSV-GPIb-BP subunits were isolated and sequenced. The deduced amino acid sequences of TSV-GPIb-BP subunits were confirmed by N-terminal protein sequencing and trypsin-digested peptide mass fingerprinting. Interestingly, the alpha subunit of TSV-GPIb-BP is identical to that of alboaggregin-B, and the sequence identity of their beta subunits is 94.3%. TSV-GPIb-BP inhibited ristocetin-induced human platelet agglutination in platelet-rich plasma under lower dosages (<5 microg/ml). On the other hand, it directly aggregated washed human platelets in the absence of additional Ca2+ or any other cofactors under higher dosages (>5 microg/ml). This platelet aggregation activity was dose-dependently inhibited by specific GPIbalpha antibodies, but not by those antibodies against platelet GPIa, GPIIa, GPIIb and GPIIIa. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the grants of ‘Western Light Project’ and ‘Tenth 5 Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M). |
| 原文出处 | 200341885.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2655]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, People’s Republic of China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, People’s Republic of China |
| 推荐引用方式 GB/T 7714 | Lee WH,Zhang Y[*]. Molecular cloning and characterization of a platelet glycoprotein Ib-binding protein from the venom of Trimeresurus stejnegeri[J]. TOXICON,2003,41(7):885-892. |
| APA | Lee WH,&Zhang Y[*].(2003).Molecular cloning and characterization of a platelet glycoprotein Ib-binding protein from the venom of Trimeresurus stejnegeri.TOXICON,41(7),885-892. |
| MLA | Lee WH,et al."Molecular cloning and characterization of a platelet glycoprotein Ib-binding protein from the venom of Trimeresurus stejnegeri".TOXICON 41.7(2003):885-892. |
入库方式： OAI收割
来源：昆明动物研究所
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