TMVA, a snake C-type lectin-like protein from Trimeresurus mucrosquamatus venom, activates platelet via GPIb
文献类型：期刊论文
| 作者 | Tai H1,2,3; Wei Q1; Jin Y1; Su M3; Song JX3; Zhou XD1,2; Ouyang HM3; Wang WY1; Xiong YL[*]1; Zhang Y1 |
| 刊名 | TOXICON
 |
| 出版日期 | 2004 |
| 卷号 | 44期号:6页码:649-656 |
| ISSN号 | 0041-0101 |
| 关键词 | Platelet aggregation TMVA GPIb Mocarhagin |
| 通讯作者 | toxinlab@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | TMVA is a C-type lectin-like protein with potent platelet activating activity from Trimeresurus mucrosquamatus venom. In the absence of von Willebrand factor (vWF), TMVA dose-dependently induced aggregation of washed platelets. Anti-GP Ib monoclonal antibodies (mAbs), HIP1, specifically inhibited TMVA-induced aggregation in a dose-dependent manner. The aggregation was also inhibited by mAb P2 (an anti-GP IIb mAb). Flow cytometric analysis revealed that FITC-TMVA bound to human formalin-fixed platelets in a saturable manner, and its binding was specifically blocked by HIP1 in a dose-dependent manner. Flow cytometric analysis showed that TMVA did not bind to platelet GPIX, GPIIb, GPIIIa, GPIa, GPIIa and GPIV. Moreover, the platelet aggregation induced by TMVA was partially inhibited when platelet was pretreated with mocarhagin, a snake venom protease that specifically cleaves human GPIb. These results suggest that TMVA is a strong platelet agonist via GPIb and it might have multiple functional binding-sites on GPIb molecule or on other unknown receptor. |
| 收录类别 | SCI |
| 原文出处 | 200444649.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2659]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_其他 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, People’s Republic of China 2.Graduate School of the Chinese Academy of Sciences, People’s Republic of China 3.The First People’s Hospital of Yunnan Province, Kunming, People’s Republic of China |
| 推荐引用方式 GB/T 7714 | Tai H,Wei Q,Jin Y,et al. TMVA, a snake C-type lectin-like protein from Trimeresurus mucrosquamatus venom, activates platelet via GPIb[J]. TOXICON,2004,44(6):649-656. |
| APA | Tai H.,Wei Q.,Jin Y.,Su M.,Song JX.,...&Zhang Y.(2004).TMVA, a snake C-type lectin-like protein from Trimeresurus mucrosquamatus venom, activates platelet via GPIb.TOXICON,44(6),649-656. |
| MLA | Tai H,et al."TMVA, a snake C-type lectin-like protein from Trimeresurus mucrosquamatus venom, activates platelet via GPIb".TOXICON 44.6(2004):649-656. |
入库方式： OAI收割
来源：昆明动物研究所
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