Purification and characterization of an irreversible serine protease inhibitor from skin secretions of Bufo andrewsi
文献类型：期刊论文
| 作者 | Zhao Y1,2; Jin Y1; Wei SS1,2; Lee WH1; Zhang Y[*]1 |
| 刊名 | TOXICON
 |
| 出版日期 | 2005 |
| 卷号 | 46期号:6页码:635-640 |
| 关键词 | Serine protease inhibitor Bufo andrewsi Skin secretions |
| ISSN号 | 0041-0101 |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 英文摘要 | Amphibian skin secretions contain many bioactive compounds. In the present work, an irreversible serine protease inhibitor, termed baserpin, was purified for the first time from the skin secretions of toad Bufo andrewsi by successive ion-exchange and gel-filtration chromatography. Baserpin is a single chain glycoprotein, with an apparent molecular weight of about 60 kDa in SDS-PAGE. Baserpin is an irreversible inhibitor and effectively inhibits the catalytic activity of trypsin, chymotrypsin and elastase. SDS-stable baserpin-trypsin complex could be seen in SDS-PAGE indicates that it possibly belongs to the serpin superfamily. According to the association rates determined, baserpin is a potent inhibitor of bovine trypsin (4.6 x 10(6) M(-1) s(-1)), bovine chymotrypsin (8.9 x 10(6) M(-1) s(-1)) and porcine elastase (6.8 x 10(6) M(-1) s(-1)), whereas it shows no inhibitory effect on thrombin. The N-terminal sequence of baserpin is HTQYPDILIAKPXDK, which shows no similarity with other known serine protease inhibitors. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M). |
| 原文出处 | 200546635.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2667]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Zhao Y,Jin Y,Wei SS,et al. Purification and characterization of an irreversible serine protease inhibitor from skin secretions of Bufo andrewsi[J]. TOXICON,2005,46(6):635-640. |
| APA | Zhao Y,Jin Y,Wei SS,Lee WH,&Zhang Y[*].(2005).Purification and characterization of an irreversible serine protease inhibitor from skin secretions of Bufo andrewsi.TOXICON,46(6),635-640. |
| MLA | Zhao Y,et al."Purification and characterization of an irreversible serine protease inhibitor from skin secretions of Bufo andrewsi".TOXICON 46.6(2005):635-640. |
入库方式： OAI收割
来源：昆明动物研究所
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