Isolation and preliminary characterization of a 22-kDa protein with trypsin inhibitory activity from toad Bufo andrewsi skin
文献类型：期刊论文
| 作者 | Zhao Y1,2; Jin Y1; Lee WH1; Zhang Y[*]1 |
| 刊名 | TOXICON
 |
| 出版日期 | 2005 |
| 卷号 | 46期号:3页码:277-281 |
| 关键词 | Trypsin inhibitor Bufo andrewsi Skin |
| ISSN号 | 0041-0101 |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | A novel trypsin inhibitor termed BATI was purified to homogeneity from the skin extracts of toad Bufo andrewsi by successive ion-exchange, gel-filtration and reverse-phase chromatography. BATI is basic single chain glycoprotein, with apparent molecular weight of 22 kDa in SDS-PAGE. BATI is a thermal stable competitive inhibitor and effectively inhibits trypsin's catalytic activity on peptide substrate with the inhibitor constant (K(i)) value of 14 nM and shows no inhibitory effect on chymotrypsin, thrombin and elastase. The N-terminal sequence of BATI is EKDSITD, which shows no similarity with other known trypsin inhibitors. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the grants of ‘Western Light’ Projects and grants from the National Natural Science Foundation (30170195, 30470380). |
| 原文出处 | 200546277.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2671]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, People’s Republic of China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, People’s Republic of China |
| 推荐引用方式 GB/T 7714 | Zhao Y,Jin Y,Lee WH,et al. Isolation and preliminary characterization of a 22-kDa protein with trypsin inhibitory activity from toad Bufo andrewsi skin[J]. TOXICON,2005,46(3):277-281. |
| APA | Zhao Y,Jin Y,Lee WH,&Zhang Y[*].(2005).Isolation and preliminary characterization of a 22-kDa protein with trypsin inhibitory activity from toad Bufo andrewsi skin.TOXICON,46(3),277-281. |
| MLA | Zhao Y,et al."Isolation and preliminary characterization of a 22-kDa protein with trypsin inhibitory activity from toad Bufo andrewsi skin".TOXICON 46.3(2005):277-281. |
入库方式： OAI收割
来源：昆明动物研究所
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