Bm-TFF2, a trefoil factor protein with platelet activation activity from frog Bombina maxima skin secretions
文献类型：期刊论文
| 作者 | Zhang J1,2; Zhang Y1,2; Wan SG1,2; Wei SS1,2; Lee WH1; Zhang Y[*]1 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2005 |
| 卷号 | 330期号:4页码:1027-1033 |
| 关键词 | Trefoil factor Platelet activation Sequence Amphibian Bombina Bm-TFF2 |
| ISSN号 | 0006-291X |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | In mammals, trefoil factor family (TFF) proteins are involved in mucosal maintenance and repair, and they are also implicated in tumor suppression and cancer progression. A novel two domain TFF protein from frog Bombina maxima skin secretions (Bm-TFF2) has been purified and cloned. It activated human platelets in a dose-dependent manner and activation of integrin alpha(IIb)beta(3) was involved. Aspirin and apyrase did not largely reduce platelet response to Bm-TFF2 (a 30% inhibition), indicating that the aggregation is not substantially dependent on ADP and thromboxane A2 autocrine feedback. Elimination of external Ca(2+) with EGTA did not influence the platelet aggregation induced by Bm-TFF2, meanwhile a strong calcium signal (cytoplasmic Ca(2+) release) was detected, suggesting that activation of phospholipase C (PLC) is involved. Subsequent immunoblotting revealed that, unlike in platelets activated by stejnulxin (a glycoprotein VI agonist), PLCgamma2 was not phosphorylated in platelets activated by Bm-TFF2. FITC-labeled Bm-TFF2 bound to platelet membranes. Bm-TFF2 is the first TFF protein reported to possess human platelet activation activity. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the grants of ‘‘Western Light’’ Projects from The Chinese Academy of Sciences to Drs. Zhang and Lee; and grants from National Nat- ural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003 C0066M). |
| 原文出处 | 20053301027.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2677]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Zhang J,Zhang Y,Wan SG,et al. Bm-TFF2, a trefoil factor protein with platelet activation activity from frog Bombina maxima skin secretions[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,330(4):1027-1033. |
| APA | Zhang J,Zhang Y,Wan SG,Wei SS,Lee WH,&Zhang Y[*].(2005).Bm-TFF2, a trefoil factor protein with platelet activation activity from frog Bombina maxima skin secretions.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,330(4),1027-1033. |
| MLA | Zhang J,et al."Bm-TFF2, a trefoil factor protein with platelet activation activity from frog Bombina maxima skin secretions".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 330.4(2005):1027-1033. |
入库方式： OAI收割
来源：昆明动物研究所
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