Cloning of two novel P-III class metalloproteinases from Trimeresurus stejnegeri venom gland
文献类型：期刊论文
| 作者 | Wan SG1,2; Jin Y1; Lee WH1; Zhang Y[*]1 |
| 刊名 | TOXICON
 |
| 出版日期 | 2006 |
| 卷号 | 47期号:4页码:465-472 |
| 关键词 | cDNA cloning Snake venom metalloproteinase Trimeresurs stejnegeri |
| ISSN号 | 0041-0101 |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Hemorrhagic toxins are widely distributed in viperid and crotalid snake venoms. Envenomation of Trimeresurus stejnegeri, a member of Crotalidae family, caused potent systemic and local hemorrhage. Up to now, there is no report on hemorrhage toxins from this venom. In this work, we cloned two cDNAs of P-III metalloproteinase precursors, designated as stejnihagin-A and stejnihagin-B, respectively, from T. stejnegeri venom gland. Both cDNAs encode an opening reading frame of 600 amino acid residues, containing a signal sequence, a proprotein domain, a metalloproteinase domain, a disintegrin-like domain and a cystetine-rich domain. Sequence analysis suggested that these two sequences shared highest similarity to the hemorrhagic toxin HR1b from T. flavoviridis. Aligning the deduced mature protein sequences of stejnihagin-A and stejnihagin-B with other snake venom metalloproteinases (SVMPs), we observed that stejnihagin-A and stejnihagin-B, together with HR1b shared the common cysteinyl residue at the position 100 in the metalloproteinase domain. In combination with the phylogenetic analysis, we presumed that stejnihagin-A, stejnihagin-B and HR1b might constitute a novel subclass of P-III SVMPs, named P-IIIc. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30470380, 30570359) and Yunnan Science and Technology Commission (2003C0066M). |
| 原文出处 | 200647465.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2689]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Wan SG,Jin Y,Lee WH,et al. Cloning of two novel P-III class metalloproteinases from Trimeresurus stejnegeri venom gland[J]. TOXICON,2006,47(4):465-472. |
| APA | Wan SG,Jin Y,Lee WH,&Zhang Y[*].(2006).Cloning of two novel P-III class metalloproteinases from Trimeresurus stejnegeri venom gland.TOXICON,47(4),465-472. |
| MLA | Wan SG,et al."Cloning of two novel P-III class metalloproteinases from Trimeresurus stejnegeri venom gland".TOXICON 47.4(2006):465-472. |
入库方式： OAI收割
来源：昆明动物研究所
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