A snake venom metalloproteinase that inhibited cell proliferation and induced morphological changes of ECV304 cells
文献类型：期刊论文
| 作者 | Wan SG1,2; Jin Y1; Lee WH1; Zhang Y[*]1 |
| 刊名 | TOXICON
 |
| 出版日期 | 2006 |
| 卷号 | 47期号:4页码:480-489 |
| 关键词 | Metalloproteinase Apoptosis Vascular endothelial cell Snake venom |
| ISSN号 | 0041-0101 |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 英文摘要 | TSV-DM, a basic metalloproteinase with a molecular weight of 110kDa, was purified from Trimeresurus stejnegeri venom. TSV-DM degraded the Aalpha chain of fibrinogen more rapidly than the Bbeta chain in a dose dependent manner. The cDNA of TSV-DM encoded a polypeptide of 622 amino acid residues, which comprises a signal peptide, proprotein, metalloproteinase domain, spacer, disintegrin-like domain and cysteine-rich domain. The protein sequence deduced from cDNA was confirmed by peptide mass fingerprinting analysis. It is highly homologous to the members of subclass P-IIIb snake venom metalloproteinase, which comprises vascular apoptosis-inducing proteins. TSV-DM inhibited cell proliferation and induced cell morphologic changes transiently of ECV304 cells. However, DNA fragmentation and DNA content analysis demonstrated that this metalloproteinase could not induce ECV304 cells apoptosis. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30170195, 30470380 and 30570359) and Yunnan Science and Technology Commission (2003C0066M and 2005PY01-23). |
| 原文出处 | 200647480.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2691]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Wan SG,Jin Y,Lee WH,et al. A snake venom metalloproteinase that inhibited cell proliferation and induced morphological changes of ECV304 cells[J]. TOXICON,2006,47(4):480-489. |
| APA | Wan SG,Jin Y,Lee WH,&Zhang Y[*].(2006).A snake venom metalloproteinase that inhibited cell proliferation and induced morphological changes of ECV304 cells.TOXICON,47(4),480-489. |
| MLA | Wan SG,et al."A snake venom metalloproteinase that inhibited cell proliferation and induced morphological changes of ECV304 cells".TOXICON 47.4(2006):480-489. |
入库方式： OAI收割
来源：昆明动物研究所
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