Purification of a lysozyme from skin secretions of Bufo andrewsi
文献类型：期刊论文
| 作者 | Zhao Y1,2; Jin Y1; Lee WH1; Zhang Y[*]1 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY, Part C
 |
| 出版日期 | 2006 |
| 卷号 | 142期号:1-2页码:46-52 |
| 关键词 | Lysozyme cDNA Bufo andrewsi Skin secretions |
| ISSN号 | 1532-0456 |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | A novel toad lysozyme (named BA-lysozyme) was purified from skin secretions of Bufo andrewsi by a three-step chromatography procedure. BA-lysozyme is a single chain protein and the apparent molecular weight is about 15 kDa as judged by SDS-PAGE. The specific lytic activity against Micrococcus lysodeikticus of BA-lysozyme is 2.7 x 10(5) units/mg, indicating that it is a potent lysozyme. It displayed potent bactericidal activity against Staphylococcus aureus and Escherichia coli with minimal inhibitory concentrations (MIC) of 1 and 8 microM, respectively. The deduced primary structure of BA-lysozyme from cloned cDNA was confirmed by N-terminal sequencing and peptide mass fingerprinting. Its amino acid sequence shares 56.5% identity with that of chicken egg-white lysozyme. Phylogenetic analysis indicates that B. andrewsi lysozyme is closely related to that of turtle. This is the first report on the isolation and primary structure determination of amphibian lysozyme. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the grants of ‘‘Western Light’’ Projects from The Chinese Academy of Sciences to Dr. Zhang and Dr. Lee; and grants from National Natural Science Foundation (30170195, 30470380, 30570359) and Yunnan Science and Technology Commission (2003C0066M). |
| 原文出处 | 200614246.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2693]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Zhao Y,Jin Y,Lee WH,et al. Purification of a lysozyme from skin secretions of Bufo andrewsi[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY, Part C,2006,142(1-2):46-52. |
| APA | Zhao Y,Jin Y,Lee WH,&Zhang Y[*].(2006).Purification of a lysozyme from skin secretions of Bufo andrewsi.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY, Part C,142(1-2),46-52. |
| MLA | Zhao Y,et al."Purification of a lysozyme from skin secretions of Bufo andrewsi".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY, Part C 142.1-2(2006):46-52. |
入库方式： OAI收割
来源：昆明动物研究所
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