Characterization and molecular cloning of dabocetin, a potent antiplatelet C-type lectin-like protein from Daboia russellii siamensis venom
文献类型：期刊论文
| 作者 | Zhong SR1,2; Jin Y1; Wu JB1,2; Chen RQ1,2; Jia YH3; Wang WY1; Xiong YL[*]1; Zhang Y1 |
| 刊名 | TOXICON
 |
| 出版日期 | 2006 |
| 卷号 | 47期号:1页码:104-112 |
| 关键词 | SV-CTLP Platelet aggregation/agglutination cDNA sequence Daboia russellii siamensis venom |
| ISSN号 | 0041-0101 |
| 通讯作者 | zhongshurong@hotmail.com |
| 合作状况 | 其它 |
| 英文摘要 | A novel C-type lectin-like protein, dabocetin, was purified from Daboia russellii siamensis venom. On SDS-polyacrylamide gel electrophoresis, it showed a single band with an apparent molecular weight of 28 kDa and two distinct bands with the apparent molecular weights of 15.0 kDa and 14.5 kDa under non-reducing and reducing conditions, respectively. cDNA clones containing the coding sequences for dabocetin alpha and beta subunits were isolated and sequenced. The deduced protein sequences of both subunits were confirmed by N-terminal amino acid sequencing and trypsin-digested peptide mass fingerprinting. Dabocetin did not induce platelet aggregation in platelet-rich plasma. It also had little effect on the platelet aggregation induced by ADP, TMVA or stejnulxin. Whereas, dabocetin inhibited ristocetin-induced platelet agglutination in platelet-rich plasma in a dose-dependent manner with an IC50 value of 0.35 microM. Flow cytometry analysis showed that dabocetin significantly inhibited mAb SZ2 binding to platelet membrane glycoprotein Ib alpha, indicating that platelet membrane glycoprotein Ib is involved in the inhibitory effect of dabocetin on ristocetin-induced platelet agglutination. |
| 收录类别 | SCI |
| 原文出处 | 200647104.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2697]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_其他 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China 3.Faculty of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China |
| 推荐引用方式 GB/T 7714 | Zhong SR,Jin Y,Wu JB,et al. Characterization and molecular cloning of dabocetin, a potent antiplatelet C-type lectin-like protein from Daboia russellii siamensis venom[J]. TOXICON,2006,47(1):104-112. |
| APA | Zhong SR.,Jin Y.,Wu JB.,Chen RQ.,Jia YH.,...&Zhang Y.(2006).Characterization and molecular cloning of dabocetin, a potent antiplatelet C-type lectin-like protein from Daboia russellii siamensis venom.TOXICON,47(1),104-112. |
| MLA | Zhong SR,et al."Characterization and molecular cloning of dabocetin, a potent antiplatelet C-type lectin-like protein from Daboia russellii siamensis venom".TOXICON 47.1(2006):104-112. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。