Isolation and cloning of a metalloproteinase from king cobra snake venom
文献类型：期刊论文
| 作者 | Guo XX1,2; Zeng L1,2; Lee WH1; Zhang Y[*]1; Jin Y1 |
| 刊名 | TOXICON
 |
| 出版日期 | 2007 |
| 卷号 | 49期号:7页码:954-965 |
| 关键词 | Metalloproteinase Elapid Ohagin Snake venom |
| ISSN号 | 0041-0101 |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | A 50 kDa fibrinogenolytic protease, ohagin, from the venom of Ophiophagus hannah was isolated by a combination of gel filtration, ion-exchange and heparin affinity chromatography. Ohagin specifically degraded the alpha-chain of human fibrinogen and the proteolytic activity was completely abolished by EDTA, but not by PMSF, suggesting it is a metalloproteinase. It dose-dependently inhibited platelet aggregation induced by ADP, TMVA and stejnulxin. The full sequence of ohagin was deduced by cDNA cloning and confirmed by protein sequencing and peptide mass fingerprinting. The full-length cDNA sequence of ohagin encodes an open reading frame of 611 amino acids that includes signal peptide, proprotein and mature protein comprising metalloproteinase, disintegrin-like and cysteine-rich domains, suggesting it belongs to P-III class metalloproteinase. In addition, P-III class metalloproteinases from the venom glands of Naja atra, Bungarus multicinctus and Bungarus fasciatus were also cloned in this study. Sequence analysis and phylogenetic analysis indicated that metalloproteinases from elapid snake venoms form a new subgroup of P-III SVMPs. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by Grants from National Natural Science Foundation of China (30630014, 30670412, 30570359 and 30470380), Yunnan Natural Science Foundation (2006C0047Q) and the Grants of ‘‘Western Light’’ Projects |
| 原文出处 | 200749954.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2701]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Guo XX,Zeng L,Lee WH,et al. Isolation and cloning of a metalloproteinase from king cobra snake venom[J]. TOXICON,2007,49(7):954-965. |
| APA | Guo XX,Zeng L,Lee WH,Zhang Y[*],&Jin Y.(2007).Isolation and cloning of a metalloproteinase from king cobra snake venom.TOXICON,49(7),954-965. |
| MLA | Guo XX,et al."Isolation and cloning of a metalloproteinase from king cobra snake venom".TOXICON 49.7(2007):954-965. |
入库方式： OAI收割
来源：昆明动物研究所
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