Molecular characterization of L-amino acid oxidase from king cobra venom
文献类型：期刊论文
| 作者 | Jin Y1; Lee WH1; Zeng L1,2; Zhang Y[*]1 |
| 刊名 | TOXICON
 |
| 出版日期 | 2007 |
| 卷号 | 50期号:4页码:479-489 |
| 关键词 | L -amino acid oxidase Elapid Snake venom Platelet aggregation Sequence |
| ISSN号 | 0041-0101 |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | An L-amino acid oxidase from Ophiophagus hannah snake venom (Oh-LAAO) was purified by successive gel filtration, ion-exchange and heparin chromatography. Oh-LAAO did not induce platelet aggregation; however, it had potent inhibitory activity on platelet aggregation induced by ADP and U46619, but showed no effect on platelet aggregation induced by thrombin, mucetin, ristocetin and stejnulxin. By RT-PCR and 5'-RACE methods, the complete Oh-LAAO cDNA was cloned from the venom gland total RNA preparations. The cDNA sequence contains an open-reading frame (ORF) of 1476-bp, which encodes a protein of 491 amino acids comprising a signal peptide of 25 amino acids and 466-residue mature protein. The predicted protein sequence of Oh-LAAO was confirmed by N-terminal and trypsin-digested internal peptides sequencing together with peptide mass fingerprinting. cDNAs encoding for ORF of LAAOs from Bungarus fasciatus and B. multicinctus were cloned and reported in this study. In addition, partial cDNA encoding for Naja atra LAAO was also reported. Oh-LAAO shared approximately 50% protein sequence identity with other known snake venom LAAOs. Phylogenetic analysis indicated that Oh-LAAO is evolutionary distant to other snake venom LAAOs. |
| 收录类别 | SCI |
| 资助信息 | The work was supported by grants from Yunnan Natural Science Foundation (2005PY01-23, 2003C0066M, 2006C0047Q), grants from National Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and grants of ‘‘Western Light’’ Projects. We also acknowledge Yunnan Blood Center for providing human blood. |
| 原文出处 | 200750479.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2705]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Jin Y,Lee WH,Zeng L,et al. Molecular characterization of L-amino acid oxidase from king cobra venom[J]. TOXICON,2007,50(4):479-489. |
| APA | Jin Y,Lee WH,Zeng L,&Zhang Y[*].(2007).Molecular characterization of L-amino acid oxidase from king cobra venom.TOXICON,50(4),479-489. |
| MLA | Jin Y,et al."Molecular characterization of L-amino acid oxidase from king cobra venom".TOXICON 50.4(2007):479-489. |
入库方式： OAI收割
来源：昆明动物研究所
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