Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom
文献类型：期刊论文
| 作者 | He YY1,2; Liu SB1,2; Lee WH1,3; Qian JQ1,2,3,4; Zhang Y[*]1,3 |
| 刊名 | PEPTIDES
 |
| 出版日期 | 2008 |
| 卷号 | 29期号:10页码:1692-1699 |
| 关键词 | Serine proteinase inhibitor Trypsin inhibitor Chymotrypsin inhibitor Snake venom Kunitz/BPTI family polypeptide King cobra |
| ISSN号 | 0196-9781 |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Snake venom Kunitz/BPTI members are good tools for understanding of structure-functional relationship between serine proteases and their inhibitors. A novel dual Kunitz/BPTI serine proteinase inhibitor named OH-TCI (trypsin- and chymotrypsin-dual inhibitor from Ophiophagus hannah) was isolated from king cobra venom by three chromatographic steps of gel filtration, trypsin affinity and reverse phase HPLC. OH-TCI is composed of 58 amino acid residues with a molecular mass of 6339 Da. Successful expression of OH-TCI was performed as the maltose-binding fusion protein in E. coli DH5 alpha . Much different from Oh11-1, the purified native and recombinant OH-TCI both had strong inhibitory activities against trypsin and chymotrypsin although the sequence identity (74.1%) between them is very high. The inhibitor constants (K-i) of recombinant OH-TCI were 3.91 x 10(-7) and 8.46 x 10(-8) M for trypsin and chymotrypsin, respectively. To our knowledge, it was the first report of Kunitz/BPTI serine proteinase inhibitor from snake venom that had equivalent trypsin and chymotrypsin inhibitory activities. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from Chinese National Natural Science Foundation (30630014, 30570359 and 30670412) and grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’, ‘‘Western Light’’ Projects from the Chinese Academy of Sciences. |
| 原文出处 | 2008291692.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2709]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China 3.Kunming Medical College, Kunming, Yunnan 650032, China 4.Department of Anesthesiology, The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650031, China |
| 推荐引用方式 GB/T 7714 | He YY,Liu SB,Lee WH,et al. Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom[J]. PEPTIDES,2008,29(10):1692-1699. |
| APA | He YY,Liu SB,Lee WH,Qian JQ,&Zhang Y[*].(2008).Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom.PEPTIDES,29(10),1692-1699. |
| MLA | He YY,et al."Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom".PEPTIDES 29.10(2008):1692-1699. |
入库方式： OAI收割
来源：昆明动物研究所
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