Identification of novel semenogelin I-derived antimicrobial peptide from liquefied human seminal plasma
文献类型：期刊论文
| 作者 | Zhao H1,2,3; Lee WH2; Shen JH3; Li H[*]1; Zhang Y[*]2 |
| 刊名 | PEPTIDES
 |
| 出版日期 | 2008 |
| 卷号 | 29期号:4页码:505-511 |
| 关键词 | antimicrobial peptides semenogelin I seminal plasma |
| ISSN号 | 0196-9781 |
| 通讯作者 | lihong_19560707@163.com ; zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Semenogelin I (SgI) is one of the most abundant proteins in human seminal plasma. SgI plays a key role in sperm coagulation and spermatozoon immobilization. in addition, SgI and/or its proteolytic fragments are involved in regulating spermatozoon motility, capacitation and inhibin-like activity. However, little is known about the antibacterial activity of SgI-derived peptides. By a combination of ion-exchange, gel filtration and high-performance liquid chromatography, peptides from liquefied human seminal plasma from 40 healthy donors were isolated and characterized. N-terminal amino-acid sequencing and fast atom bombardment mass spectrometry revealed that four isolated peptides were SgI-derived, namely SgI-29 (8S-113), SgI-46 (85-130), SgI-47 (85-131) and SgI-52 (85-136). Interestingly, SgI-29, SgI-46 and SgI-47 are newly identified SgI-derived peptides. Antimicrobial activity assay results indicated that synthesized SgI-29 had strong antibacterial activity toward various bacterial strains. Our results indicate that SgI can be digested into small fragments like newly identified SgI-29, SgI-46 and SgI-47 and may have diversified functions. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’ and ‘‘Western Light’’ projects from The Chinese Academy of Sciences; and grants from National Natural Science Foundation (30470380, 30630014, 30670412) and Yunnan Science and Technology Commission (2005PY01-23, 2007C0006R). |
| 原文出处 | 200829505.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2717]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China 2.Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China 3.Department of Urology, The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, China |
| 推荐引用方式 GB/T 7714 | Zhao H,Lee WH,Shen JH,et al. Identification of novel semenogelin I-derived antimicrobial peptide from liquefied human seminal plasma[J]. PEPTIDES,2008,29(4):505-511. |
| APA | Zhao H,Lee WH,Shen JH,Li H[*],&Zhang Y[*].(2008).Identification of novel semenogelin I-derived antimicrobial peptide from liquefied human seminal plasma.PEPTIDES,29(4),505-511. |
| MLA | Zhao H,et al."Identification of novel semenogelin I-derived antimicrobial peptide from liquefied human seminal plasma".PEPTIDES 29.4(2008):505-511. |
入库方式： OAI收割
来源：昆明动物研究所
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