Decreased expression of protease-activated receptor 4 in human gastric cancer
文献类型：期刊论文
| 作者 | Zhang Y1; Yu GY1,2; Jiang P1,3; Xiang Y1; Li WL4; Lee WH1; Zhang Y[*]1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 43期号:9页码:1277-1283 |
| 关键词 | Protease-activated receptor Gastric cancer Real-time PCR Immunohistochemistry Methylation |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Protease-activated receptors (PARs) are a unique family of G-protein coupled receptors. PAR4, the most recently identified PAR member, was reported to be overexpressed during the progression of colon and prostate cancers. Though PAR4 mRNA was detected in normal stomach, the role of PAR4 in gastric cancer has not been investigated. In this study, differential expression of PAR4 was measured by real-time PCR (n = 28) and tissue microarrays (n = 74). We showed that PAR4 was located from basal to middle portions of normal gastric mucosa. PAR4 expression was remarkably decreased in gastric cancer tissues as compared with matched noncancerous tissues, especially in positive lymph node or low differentiation cancers. Furthermore, methylation of the PAR4 promoter in cell lines was assessed by treatment with 5-aza-2'-deoxycytidine and genomic bisulfite sequencing. AGS and N87 human gastric cancer cell lines did not express PAR4, as compared to HT-29 human colon cancer cell line with significant PAR4 expression. Treatment with 5-aza-2'-deoxycytidine restored PAR4 expression in AGS and N87 cells, which exhibited significantly more 5-methylcytosines in the PAR4 promoter compared with HT-29 cells. Our results revealed that down-regulation of PAR4 expression occurs frequently in gastric cancers and exhibits association with more aggressive gastric cancer. Interestingly, the loss of PAR4 expression in gastric cancers may result from hypermethylation of the PAR4 promoter. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from National Basic Research Program of China (973 Program, 2010CB529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese Academy of Sciences “Key Research Direction” (KSCX2-YW-R-088) and “Western Light Project” (Y102291081). |
| 语种 | 英语 |
| 公开日期 | 2011-09-05 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6751]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China 2.Department of Biochemistry, Kunming Medical College, Kunming, Yunnan 650032, China 3.Department of Pathology, Kunming Medical College, Kunming, Yunnan 650032, China 4.Department of Gastroenterology, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, China |
| 推荐引用方式 GB/T 7714 | Zhang Y,Yu GY,Jiang P,et al. Decreased expression of protease-activated receptor 4 in human gastric cancer[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2011,43(9):1277-1283. |
| APA | Zhang Y.,Yu GY.,Jiang P.,Xiang Y.,Li WL.,...&Zhang Y[*].(2011).Decreased expression of protease-activated receptor 4 in human gastric cancer.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,43(9),1277-1283. |
| MLA | Zhang Y,et al."Decreased expression of protease-activated receptor 4 in human gastric cancer".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 43.9(2011):1277-1283. |
入库方式： OAI收割
来源：昆明动物研究所
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