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Extremely Abundant Antimicrobial Peptides Existed in the Skins of Nine Kinds of Chinese Odorous Frogs
文献类型：期刊论文
| 作者 | Yang XW1,2; Lee WH[*]1; Zhang Y[*]1 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2012 |
| 卷号 | 11期号:1页码:306-319 |
| 关键词 | AMPs extreme diversity odorous frogs |
| 通讯作者 | leewh@mail.kiz.ac.cn ; zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Peptide agents are regarded as hopeful candidates to solve life-threatening resistance of pathogenic microorganisms to classic antibiotics due to their unique action mechanisms. Peptidomic and genomic investigation of natural antimicrobial peptides (AMPs) from amphibian skin secretions can provide a large amount of structure-functional information to design peptide antibiotics with therapeutic potential. In the present study, we identified a large number of AMPs from the skins of nine kinds of Chinese odorous frogs. Eighty AMPs were purified from three different odorous frogs and confirmed by peptidomic analysis. Our results indicated that post-translational modification of AMPs rarely happened in odorous frogs. cDNAs encoding precursors of 728 AMPs, including all the precursors of the confirmed 80 native peptides, were cloned from the constructed AMP cDNA libraries of nine Chinese odorous frogs. On the basis of the sequence similarity of deduced mature peptides, these 728 AMPs were grouped into 97 different families in which 71 novel families were identified. Out of these 728 AMPs, 662 AMPs were novel and 28 AMPs were reported previously in other frog species. Our results revealed that identical AMPs were widely distributed in odorous frogs; 49 presently identified AMPs could find their identical molecules in different amphibian species. Purified peptides showed strong antimicrobial activities against 4 tested microbe strains. Twenty-three deduced peptides were synthesized and their bioactivities, including antimicrobial, antioxidant, hemolytic, immunomodulatory and insulin-releasing activities, were evaluated. Our findings demonstrate the extreme diversity of AMPs in amphibian skins and provide plenty of templates to develop novel peptide antibiotics, |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from National Basic Research Program of China (973 Program, 2010CB529800) and The National NaturalScienceFoundation ofChina (NSFC- Yunnan-Union-Funding U1132601, and 31071926, 30870304). |
| 语种 | 英语 |
| WOS记录号 | WOS:000298827700028 |
| 公开日期 | 2012-03-20 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6896]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China |
| 推荐引用方式 GB/T 7714 | Yang XW,Lee WH[*],Zhang Y[*]. Extremely Abundant Antimicrobial Peptides Existed in the Skins of Nine Kinds of Chinese Odorous Frogs[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(1):306-319. |
| APA | Yang XW,Lee WH[*],&Zhang Y[*].(2012).Extremely Abundant Antimicrobial Peptides Existed in the Skins of Nine Kinds of Chinese Odorous Frogs.JOURNAL OF PROTEOME RESEARCH,11(1),306-319. |
| MLA | Yang XW,et al."Extremely Abundant Antimicrobial Peptides Existed in the Skins of Nine Kinds of Chinese Odorous Frogs".JOURNAL OF PROTEOME RESEARCH 11.1(2012):306-319. |
入库方式： OAI收割
来源：昆明动物研究所
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