Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis
文献类型：期刊论文
| 作者 | Chen ZM1,2; Yang XW1,3; Liu ZC1,3; Zeng L1,3; Lee WH1; Zhang Y[*]1 |
| 刊名 | BIOCHIMIE
 |
| 出版日期 | 2012 |
| 卷号 | 94期号:2页码:328-334 |
| 关键词 | Antimicrobial peptide Jindongenin-1a Palustrin-2AJ1/2 Multi-activities Amolops jingdongensis |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | The characterization of new natural antimicrobial peptides (AMPs) can help to solve the serious problem of bacterial resistance to currently used antibiotics. In the current study, we analyzed two families of AMPs from the Chinese torrent frog Amolops jingdongensis with a range of bioactivities. The first family of peptides, named jindongenin-1a, is 24 amino acids in length; a BLAST search of jindongenin-1a revealed no sequence similarity with other AMPs. The second family consists of two peptides containing 29 amino acid residues each. These peptides have high sequence similarity with the AMPs of palustrin-2 and are therefore designated palustrin-2AJ1 and palustrin-2AJ2. The cDNA sequences encoding these AMPs have been cloned and the deduced protein sequence of each AMP has been determined by protein sequencing. Sequence and structural analysis showed that each precursor is composed of a putative signal peptide, an N-terminal spacer, a processing site and a disulfide-bridged heptapeptide segment at the C-terminus. We synthesized jindongenin-1a and palustrin-AJ1 to test their antimicrobial, hemolytic, antioxidative and cytotoxic activities. These two peptides showed broad-spectrum antimicrobial activity to standard and clinically isolated strains of bacteria. In addition, they exhibited weak hemolytic activity to human and rabbit erythrocytes under our experimental conditions. Moreover, these peptides also displayed cytotoxic activity against the K562 and HT29 mammalian cell lines and low anti-oxidant activity. These findings provide helpful insight that will be useful in the design of anti-infective peptide agents. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), the Chinese NationalNatural Science Foundation (30630014, 30870304 and 31071926). |
| 语种 | 英语 |
| WOS记录号 | WOS:000300270900007 |
| 公开日期 | 2012-04-05 |
| 源URL | [http://159.226.149.42:8088/handle/152453/6902]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223, Yunnan, China 2.Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650224, Yunan, China 3.The Graduate School of the Chinese Academy of Sciences, Beijing 100009, China |
| 推荐引用方式 GB/T 7714 | Chen ZM,Yang XW,Liu ZC,et al. Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis[J]. BIOCHIMIE,2012,94(2):328-334. |
| APA | Chen ZM,Yang XW,Liu ZC,Zeng L,Lee WH,&Zhang Y[*].(2012).Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis.BIOCHIMIE,94(2),328-334. |
| MLA | Chen ZM,et al."Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis".BIOCHIMIE 94.2(2012):328-334. |
入库方式： OAI收割
来源：昆明动物研究所
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