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Venomic and Transcriptomic Analysis of Centipede Scolopendra subspinipes dehaani

文献类型：期刊论文

作者Liu ZC1,3; Zhang R2; Zhao F1,3; Chen ZM1; Liu HW2; Wang YJ1; Jiang P1; Zhang Y1; Wu Y2; Ding JP[*]2
刊名JOURNAL OF PROTEOME RESEARCH
出版日期2012
卷号11期号:12页码:6197-6212
关键词centipede proteomic Scolopendra transcriptomic venom
通讯作者jpding@mail.hust.edu.cn ; leewh@mail.kiz.ac.cn ; zhangy@mail.kiz.ac.cn
合作状况其它
英文摘要   Centipedes have venom glands in their first pair of limbs, and their venoms contain a large number of components with different biochemical and pharmacological properties. However, information about the compositions and functions of their venoms is largely unknown. In this study, Scolopendra subspinipes dehaani venoms were systematically investigated by transcriptomic and proteomic analysis coupled with biological function assays. After random screening approximately 1500 independent clones, 1122 full length cDNA sequences, which encode 543 different proteins, were cloned from a constructed cDNA library using a pair of venom glands from a single centipede species. Neurotoxins, ion channel acting components and venom allergens were the main fractions of the crude venom as revealed by transcriptomic analysis. Meanwhile, 40 proteins/peptides were purified and characterized from crude venom of S. subspinipes dehaani. The N-terminal amino acid sequencing and mass spectrum results of 29 out of these 40 proteins or peptides matched well With their corresponding cDNAs. The purified proteins/peptides showed different pharmacological properties, including the following: (1) platelet aggregating activity; (2) anticoagulant activity; (3) phospholipase A(2) activity; (4) trypsin inhibiting activity; (5) voltage gated potassium channel activities; (6) voltage gated sodium channel activities; (7) voltage gated calcium channel activities. Most of them showed no significant similarity to other protein sequences deposited in the known public database. This work provides the largest number of protein or peptide candidates with medical-pharmaceutical significance and reveals the toxin nature of centipede S. subspinipes dehaani venom.
资助信息This work was supported by grants from National Basic Research Program of China (973 Program, 2010CB529800) and The National Natural Science Foundation of China (NSFC-Yunnan-Union-Funding U1132601, 31071926 and 31270835). Y206A41181 from Kunming Institute of Zoology and 2011CI139 from Yunnan Province are also acknowledged.
收录类别SCI
语种英语
WOS记录号WOS:000311925900054
公开日期2013-09-18
源URL[http://159.226.149.42:8088/handle/152453/7651]  
专题昆明动物研究所_动物活性蛋白多肽组学
昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, Yunnan 650223, China
2.Key Laboratory of Molecular Biophysics, Huazhong University of Science and Technology, the Ministry of Education, Wuhan, Hubei 430074, China
3.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
推荐引用方式
GB/T 7714		 Liu ZC,Zhang R,Zhao F,et al. Venomic and Transcriptomic Analysis of Centipede Scolopendra subspinipes dehaani[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(12):6197-6212.
APA Liu ZC.,Zhang R.,Zhao F.,Chen ZM.,Liu HW.,...&Zhang Y[*].(2012).Venomic and Transcriptomic Analysis of Centipede Scolopendra subspinipes dehaani.JOURNAL OF PROTEOME RESEARCH,11(12),6197-6212.
MLA Liu ZC,et al."Venomic and Transcriptomic Analysis of Centipede Scolopendra subspinipes dehaani".JOURNAL OF PROTEOME RESEARCH 11.12(2012):6197-6212.

入库方式： OAI收割

来源：昆明动物研究所

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