Differential expression and subcellular localization of Prohibitin 1 are related to tumorigenesis and progression of non-small cell lung cancer
文献类型：期刊论文
| 作者 | Jiang P1,2,3; Xiang Y1; Wang YJ1; Li SM4; Wang Y5; Hua HR3; Yu GY5; Zhang Y1; Lee WH1; Zhang Y[*]1 |
| 刊名 | INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 6期号:10页码:2092-2101 |
| 关键词 | Prohibitin 1 up-regulation subcellular localization non-small cell lung cancer |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 英文摘要 | Lung cancer remains the leading cause of cancer-related deaths worldwide and non-small cell lung cancer (NSCLC) accounts for approximately 85% of all lung cancer. With a variety of biological functions, Prohibitin1 (PHB1) has been proved tumor-associated. But there are conflicting data regarding the involvement of PHB1 in tumorigenesis and few studies regarding the role of PHB1 in lung cancer. The studies reported herein used a combination of clinical observations and molecular methods to investigate the possible role of PHB1 in NSCLC tissues and cell lines. PHB1 expression was evaluated by RT-PCR, RT-qPCR, Western blotting and immunohistochemistry analysis. Flow cytometric analysis was used to determine the surface expression profiles of PHB1 in lung cell lines. The results showed that PHB1 expression were generally increased in lung cancer tissues when compared with matched noncancerous tissues and closely related with tumor differentiation and lymph node invasion. PHB1 expression levels was also increased in three lung cancer cell lines (SK-MES-1, NCI-H157 and NCI-H292) as compared with BEAS-2B cells. Moreover, there were various subcellular localization of PHB1 in different lung cancer cells and the presence of PHB1 on the surface of lung cancer cells was significantly reduced. In conclusion, PHB1 expression is increased in NSCLC and the up-regulation of PHB1 is associated with clinically aggressive phenotype. The different subcellular localization of PHB1 in NSCLC cells and the loss of the membrane-associated PHB1 probably related to the tumorigenesis and progression of NSCLC and suggests that PHB1 may play different roles in various types of NSCLC |
| 收录类别 | SCI |
| 资助信息 | This work is supported by grants from the Chinese National Natural Science Foundation (31270835, U1132601), the National Basic Research Program of China (973 Program, 2010CB529800), and the Key Research Pro- gram of the Chinese Academy of Sciences (KJZD-EW-L03). |
| 语种 | 英语 |
| WOS记录号 | WOS:000328250800014 |
| 公开日期 | 2014-01-03 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7767]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of The Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.University of Chinese Academy of Sciences, Beijing 100049, China 3.Department of Pathology and Pathophysi- ology, Kunming Medical University, Kunming, Yunnan 650500, China 4.Department of Biochemistry, Kunming Medical University, Kunming, Yunnan 650500, China 5.5 Department of Pathology, The Secondly Affiliated Hospital of Kunming Medical University, Kunming 650032, China |
| 推荐引用方式 GB/T 7714 | Jiang P,Xiang Y,Wang YJ,et al. Differential expression and subcellular localization of Prohibitin 1 are related to tumorigenesis and progression of non-small cell lung cancer[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2013,6(10):2092-2101. |
| APA | Jiang P.,Xiang Y.,Wang YJ.,Li SM.,Wang Y.,...&Zhang Y[*].(2013).Differential expression and subcellular localization of Prohibitin 1 are related to tumorigenesis and progression of non-small cell lung cancer.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,6(10),2092-2101. |
| MLA | Jiang P,et al."Differential expression and subcellular localization of Prohibitin 1 are related to tumorigenesis and progression of non-small cell lung cancer".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 6.10(2013):2092-2101. |
入库方式： OAI收割
来源：昆明动物研究所
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