Promoter hypermethylation and downregulation of trefoil factor 2 in human gastric cancer
文献类型：期刊论文
| 作者 | Jiang P1,2; Yu GY1,3; Zhang Y[*]1; Xiang YL1; Zhu Z4; Feng WY3; Lee WH1; Zhang Y[*]1 |
| 刊名 | ONCOLOGY LETTERS
 |
| 出版日期 | 2014 |
| 卷号 | 7期号:5页码:1525-1531 |
| 关键词 | trefoil factor 2 downregulation gastric cancer promoter hypermethylation |
| 通讯作者 | zhyong@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Trefoil factor 2 (TFF2) plays a protective role in gastric mucosa and may be involved in the progression of gastric cancer, but the detailed functions and underlying molecular mechanisms are not clear. The present study used a combination of clinical observations and molecular methods to investigate the correlation between abnormal expression of TFF2 and gastric cancer progression. TFF2 expression was evaluated by reverse transcription polymerase chain reaction (RT-PCR), quantitative PCR (qPCR), and western blot and immunohistochemistry analyses. TFF2 methylation levels were analyzed by genomic bisulfite sequencing method. The results showed that TFF2 mRNA and protein expression were decreased in gastric cancer tissues compared with the matched non-cancerous mucosa, and the decreased level was associated with the differentiation and invasion of gastric cancer. Moreover, the average TFF2 methylation level of CpG sites in the promoter region was 70.4% in three gastric cancer tissues, while the level in associated non-neoplastic tissues was 41.0%. Furthermore, the promoter hypermethylation of TFF2 was also found in gastric cancer cell lines, AGS and N87, and gene expression was significantly increased following treatment with a demethylating agent, 5-Aza-2'-deoxycytidine. In conclusion, TFF2 expression was markedly decreased in gastric cancer and promoter hypermethylation was found to regulate the down regulation of TFF2. TFF2 has been suggested as a tumor suppressor in gastric carcinogenesis and metastasis. |
| 收录类别 | SCI |
| 资助信息 | The current study was supported by grants from the Chinese National Natural Science Foundation (nos. 81160302, 31270835 and 31201720), NSFC Yunnan Union Funding (no. U113 2601), the Science and Technology Project of Yunnan Province (no. 2011FZ109) and the Chinese Academy of Sciences ‘Western Light Project’ (no. Y102291081). |
| 语种 | 英语 |
| WOS记录号 | WOS:000337558000038 |
| 公开日期 | 2014-10-15 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8048]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223 2.Pathology and Pathophysiology,Kunming Medical University, Kunming, Yunnan 650500 3.Biochemistry, Kunming Medical University, Kunming, Yunnan 650500 4.Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming,Yunnan 650032, P.R. China |
| 推荐引用方式 GB/T 7714 | Jiang P,Yu GY,Zhang Y[*],et al. Promoter hypermethylation and downregulation of trefoil factor 2 in human gastric cancer[J]. ONCOLOGY LETTERS,2014,7(5):1525-1531. |
| APA | Jiang P.,Yu GY.,Zhang Y[*].,Xiang YL.,Zhu Z.,...&Zhang Y[*].(2014).Promoter hypermethylation and downregulation of trefoil factor 2 in human gastric cancer.ONCOLOGY LETTERS,7(5),1525-1531. |
| MLA | Jiang P,et al."Promoter hypermethylation and downregulation of trefoil factor 2 in human gastric cancer".ONCOLOGY LETTERS 7.5(2014):1525-1531. |
入库方式： OAI收割
来源：昆明动物研究所
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