Betagamma-CAT, a non-lens betagamma-crystallin and trefoil factor complex from amphibian skin secretions, caused endothelium-dependent myocardial depression in isolated rabbit hearts
文献类型：期刊论文
| 作者 | Qian JQ1,2,3; Liu SB1,3; He YY1,3; Lee WH1; Zhang Y[*]1 |
| 刊名 | TOXICON
 |
| 出版日期 | 2008 |
| 卷号 | 52期号:2页码:285-292 |
| 关键词 | tumor necrosis factor-alpha trefoil factor non-lens beta gamma-crystallin beta gamma-CAT cardiac dysfunction heart failure |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Previous in vivo study demonstrated that beta gamma-CAT, a newly identified non-lens beta gamma-crystallin and trefoil factor complex from frog Bombina maxima skin secretions, possessed potent lethal toxicity on mammals resulted from hypotension and cardiorespiratory arrest. However, the mechanism of cardiac dysfunction induced by the protein is unknown. Here, we report that beta gamma-CAT, with dosages of 0.8-3.0 nM, elicited an acute negative inotropic effect in isolated rabbit heart Langensdorff preparations, which mimicked acute heart failure. In addition, the effect of beta gamma-CAT on the hearts was mediated by endothelium-dependent coronary vasoconstriction (P < 0.01, compared between endothelium-intact and removal hearts). After beta gamma-CAT (3.0 nM) treatment, the positive signal of tumor necrosis factor-alpha (TNF-alpha) was detected mainly around the endothelial cell layer as detected by in situ indirect immunofluorescence, indicating that the release of TNF-a occurred. At the same time, a rapid TNF-a release was detected in primary cultured rabbit endocardial endothelial cells (REECs) treated with fly-CAT. After addition of beta gamma-CAT (3.0 nM) for 10 min and 30 min, the TNIF-alpha levels were increased to 57.33 +/- 3.22 pg/ml and 60.00 +/- 5.35 pg/ml (P < 0.05, compared with the control values of 21.67 +/- 3.45 pg/ml and 33.70 +/- 6.24 pg/.ml, respectively). At high concentrations, beta gamma-CAT interfered with the cell viability of REECs (CC50 about 25 nM). Taken together, beta gamma-CAT was able to induce acute myocardial depression and the toxic effect might be partially explained by the release of TNF-alpha. The finding provides new information to understand the patho-physiological roles of non-lens beta gamma-crystallins and trefoil factors. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the grants from The Chi- nese National Natural Science Foundation (30630014 and 30570359) and the grants of ‘‘Key Research Direction- KSCX2-YW-R-088’’ from The Chinese Academy of Sciences and Yunnan Provincial Natural Science Foundation (2006PT08-1) to Yun Zhang. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9624]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China 2.Department of Anesthesiology, The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Qian JQ,Liu SB,He YY,et al. Betagamma-CAT, a non-lens betagamma-crystallin and trefoil factor complex from amphibian skin secretions, caused endothelium-dependent myocardial depression in isolated rabbit hearts[J]. TOXICON,2008,52(2):285-292. |
| APA | Qian JQ,Liu SB,He YY,Lee WH,&Zhang Y[*].(2008).Betagamma-CAT, a non-lens betagamma-crystallin and trefoil factor complex from amphibian skin secretions, caused endothelium-dependent myocardial depression in isolated rabbit hearts.TOXICON,52(2),285-292. |
| MLA | Qian JQ,et al."Betagamma-CAT, a non-lens betagamma-crystallin and trefoil factor complex from amphibian skin secretions, caused endothelium-dependent myocardial depression in isolated rabbit hearts".TOXICON 52.2(2008):285-292. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。