A novel heme-containing protein with anti-HIV-1 activity from skin secretions of Bufo andrewsi
文献类型：期刊论文
| 作者 | Zhao Y1,3; Jin Y1; Lee WH1; Zheng YT[*]2; Zhang Y[*]1; Wang JH2,3; Wang RR2,3; Yang LM2 |
| 刊名 | Toxicon
 |
| 出版日期 | 2005 |
| 卷号 | 46期号:6页码:619–624 |
| 关键词 | Toad Skin secretion HIV-1 Heme Bufo andrewsi |
| 通讯作者 | zhangyt@mail.kiz.ac.cn ; zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | A novel protein, named BAS-AH, was purified and characterized from the skin of the toad Bufo andrewsi. BAS-AH is a single chain protein and the apparent molecular weight is about 63 kDa as judged by SDS-PAGE. BAS-AH was determined to bind heme (0.89 mol heme/mol protein) as determined by pyridine haemochrome analysis. Fifty percentage cytotoxic concentration (CC50) of BAS-AH on C8166 cells was 9.5 microM. However, at concentrations that showed little effect on cell viability, BAS-AH displayed dose dependent inhibition on HIV-1 infection and replication. The antiviral selectivity indexes (CC50/EC50) were 14.4 and 11.4, respectively, corresponding to the measurements of syncytium formation and HIV-1 p24 antigen expression. BAS-AH also showed an inhibitory effect on the activity of recombinant HIV-1 reverse transcriptase (IC50 = 1.32 microM). The N-terminal sequence of BAS-AH was determined to be NAKXKADVIGKISILLGQDNLSNIVAAM, which exhibited little identity with other known anti-HIV-1 proteins. BAS-AH is devoid of antibacterial, proteolytic, trypsin inhibitory activity, l-amino acid oxidase activity and catalase activity. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M) to Dr Y Zhang, CAS Knowledge Innovation Projects (KSCX2- SW-216), Key Scientific and Technological projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142) to Dr Y.T. Zheng |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9652]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_分子免疫药理学 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China 2.Laboratory of Molecular Immunopharmacology, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Zhao Y,Jin Y,Lee WH,et al. A novel heme-containing protein with anti-HIV-1 activity from skin secretions of Bufo andrewsi[J]. Toxicon,2005,46(6):619–624. |
| APA | Zhao Y.,Jin Y.,Lee WH.,Zheng YT[*].,Zhang Y[*].,...&Yang LM.(2005).A novel heme-containing protein with anti-HIV-1 activity from skin secretions of Bufo andrewsi.Toxicon,46(6),619–624. |
| MLA | Zhao Y,et al."A novel heme-containing protein with anti-HIV-1 activity from skin secretions of Bufo andrewsi".Toxicon 46.6(2005):619–624. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。