树鼩生化指标正常值测定和三叶因子原核表达 载体构建
文献类型：学位论文
| 作者 | 马旭通 |
| 学位类别 | 硕士 |
| 答辩日期 | 2011-05 |
| 授予单位 | 中国科学院研究生院 |
| 授予地点 | 北京 |
| 导师 | 张云 |
| 关键词 | 树鼩 血液样本 生理指标 三叶因子 表达 |
| 其他题名 | Detection of biochemical indexes in tree shrew and construction of prokaryotic expression vector of tree shrew TFF2 |
| 学位专业 | 生物化学与分子生物学 |
| 中文摘要 | 鉴于树鼩独特的分类学地位及解剖学、生理学特性, 树鼩已应用于生物医学研究的诸多领域，而且研究中必须具有完备的生理生化指标等基础数据。鉴于之前的基础数据来源于野生树鼩以及当前实验室树鼩繁育的推广，本文得到了野生树鼩和实验室繁育树鼩的23项生化指标，并且将得到的结果和已发表论文上的结果进行比较。本文的数据和比较结果为将来树鼩的研究提供了依据。 树鼩具有明显的易激特征，存在作为胃肠道溃疡等粘膜性疾病模型动物的潜在可能。树鼩TFF的表达将为进一步的相关研究提供必要地研究基础。本文克隆了树鼩三叶因子TFF2，并且构建了原核表达载体pET32a-tsTFF2。将获得的cDNA序列运行NCBI的程序BLASTn，返回的核酸序列仅限于灵长类动物；用翻译后的蛋白序列进行BLASTp后，得到包括灵长类、啮齿类等的很多蛋白序列，其中与小鼠的序列一致性（85.8%）甚至高于人（83%）。据此可以推测，树鼩的分类地位更加接近灵长类，而TFF2的氨基酸序列非常保守。 |
| 英文摘要 | With their unique taxonomic status and anatomical, physiological characteristics, tree shrews have been used as animal models of human diseases for a long time. It is essential to have enough basic data including biochemical indexes. In view of the basic data derived from wild tree shrews and the development of laboratory tree shrews, 23 biochemical indexes of wild tree shrews and laboratory tree shrews are studied in this article. Some of them are compared with those from published papers. As tree shrews are excitable, it is possible to be developed an animal model with mucosal disease such as gastrointestinal ulcers. The expression of TFF is fundamental to the subsequent research. TFF2 of tree shrews is cloned and its prokaryotic expression vector ( pET32a-tsTFF2) is cloned. When the sequence of tree shrews TFF2 cDNA was submitted to the progress BLASTn on NCBI, the query sequences are all from primates, while the corresponding protein sequence was submitted, the query sequences are wildly distributed, including primates, redents and so on. The percentage of identity is much higher in tree shrews TFF2 and mouse TFF2 than that in tree shrews TFF2 and human. It could be speculated that the taxonomic status of the tree shrew is closer to primates than others, and the amino acid sequence of TFF2 is very conservative. |
| 语种 | 中文 |
| 公开日期 | 2013-04-23 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7374]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 |
| 推荐引用方式 GB/T 7714 | 马旭通. 树鼩生化指标正常值测定和三叶因子原核表达 载体构建[D]. 北京. 中国科学院研究生院. 2011. |
入库方式： OAI收割
来源：昆明动物研究所
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