A Single Streptomyces Symbiont Makes Multiple Antifungals to Support the Fungus Farming Ant Acromyrmex octospinosus
文献类型：期刊论文
| 作者 | Seipke RF[*]1; Barke J1; Brearley C1; Hill L2; Yu DW1,3; Goss RJM4; Hutchings MI[*]1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2011 |
| 卷号 | 6期号:8页码:e22028 |
| 通讯作者 | r.seipke@uea.ac.uk |
| 合作状况 | 其它 |
| 英文摘要 | Attine ants are dependent on a cultivated fungus for food and use antibiotics produced by symbiotic Actinobacteria as weedkillers in their fungus gardens. Actinobacterial species belonging to the genera Pseudonocardia, Streptomyces and Amycolatopsis have been isolated from attine ant nests and shown to confer protection against a range of microfungal weeds. In previous work on the higher attine Acromyrmex octospinosus we isolated a Streptomyces strain that produces candicidin, consistent with another report that attine ants use Streptomyces-produced candicidin in their fungiculture. Here we report the genome analysis of this Streptomyces strain and identify multiple antibiotic biosynthetic pathways. We demonstrate, using gene disruptions and mass spectrometry, that this single strain has the capacity to make candicidin and multiple antimycin compounds. Although antimycins have been known for > 60 years we report the sequence of the biosynthetic gene cluster for the first time. Crucially, disrupting the candicidin and antimycin gene clusters in the same strain had no effect on bioactivity against a co-evolved nest pathogen called Escovopsis that has been identified in similar to 30% of attine ant nests. Since the Streptomyces strain has strong bioactivity against Escovopsis we conclude that it must make additional antifungal(s) to inhibit Escovopsis. However, candicidin and antimycins likely offer protection against other microfungal weeds that infect the attine fungal gardens. Thus, we propose that the selection of this biosynthetically prolific strain from the natural environment provides A. octospinosus with broad spectrum activity against Escovopsis and other microfungal weeds. |
| 收录类别 | SCI |
| 资助信息 | This work was funded by the Medical Research Council Milstein Award (grant G0801721) to MIH, RJMG and DWY. |
| 语种 | 英语 |
| 公开日期 | 2011-09-05 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6752]  |
| 专题 | 昆明动物研究所_动物生态学研究中心 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, United Kingdom 2.Metabolic Biology, John Innes Centre, Norwich Research Park, Norwich, United Kingdom 3.State Key Laboratory of Genetic Resources, and Evolution, Ecology, Conservation, and Environment Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 4.School of Chemistry, University of East Anglia, Norwich Research Park, Norwich, United Kingdom |
| 推荐引用方式 GB/T 7714 | Seipke RF[*],Barke J,Brearley C,et al. A Single Streptomyces Symbiont Makes Multiple Antifungals to Support the Fungus Farming Ant Acromyrmex octospinosus[J]. PLOS ONE,2011,6(8):e22028. |
| APA | Seipke RF[*].,Barke J.,Brearley C.,Hill L.,Yu DW.,...&Hutchings MI[*].(2011).A Single Streptomyces Symbiont Makes Multiple Antifungals to Support the Fungus Farming Ant Acromyrmex octospinosus.PLOS ONE,6(8),e22028. |
| MLA | Seipke RF[*],et al."A Single Streptomyces Symbiont Makes Multiple Antifungals to Support the Fungus Farming Ant Acromyrmex octospinosus".PLOS ONE 6.8(2011):e22028. |
入库方式： OAI收割
来源：昆明动物研究所
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