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Using metabarcoding to ask if easily collected soil and leaf-litter samples can be used as a general biodiversity indicator

文献类型：期刊论文

作者Yang CX1,2; Wang XY1; Miller JA3; de Blecourt M4; Ji YQ1; Yang CY1; Harrison RD5; Yu DW[*]1,6
刊名ECOLOGICAL INDICATORS
出版日期2014
卷号46期号:X页码:379-389
关键词Soil fauna Leaf litter DNA barcoding Biodiversity Restoration ecology
通讯作者dougwyu@gmail.com
合作状况其它
英文摘要The targeted sequencing of taxonomically informative genetic markers, sometimes known as metabarcoding, allows eukaryote biodiversity to be measured rapidly, cheaply, comprehensively, repeatedly, and verifiably. Metabarcoding helps to remove the taxonomic impediment, which refers to the great logistical difficulties of describing and identifying species, and thus promises to improve our ability to detect and respond to changes in the natural environment. Now, sampling has become a rate-limiting step in biodiversity measurement, and in an effort to reduce turnaround time, we use arthropod samples from southern China and Vietnam to ask whether soil, leaf litter, and aboveground samples provide similar ecological information. A soil or leaf-litter sample can be collected in minutes, whereas an aboveground sample, such as from Malaise traps or canopy fogging, can require days to set up and run, during which time they are subject to theft, damage, and deliberate contamination. Here we show that while the taxonomic compositions of soil and leaf-litter samples are very different from aboveground samples, both types of samples provide similar ecological information, in terms of ranking sites by species richness and differentiating sites by beta diversity. In fact, leaf-litter samples appear to be as or more powerful than Malaise-trap and canopy-fogging samples at detecting habitat differences. We propose that metabarcoded leaf-litter and soil samples be widely tested as a candidate method for rapid environmental monitoring in terrestrial ecosystems
收录类别SCI
资助信息the German Agency for International Cooperation(G.I.Z.)andtheGermanMinistryforEconomicCooper- ation(B.M.Z.)projectMMC(MakingtheMekongConnected,Project No. 08.7860.3-001.00). Y.C.X., W.X.Y., J.Y.Q., C.Y.Y., and D.W.Y. were supportedbyYunnanProvince(20080A001),theChineseAcademy of Sciences (0902281081, KSCX2-YW-Z-1027), the National Natu- ralScienceFoundationofChina(31170498),theMinistryofScience and Technology of the People’s Republic of China (2012FY110800), the University of East Anglia, and the State Key Laboratory of Genetic Resources and Evolution at the Kunming Institute of Zool- ogy.
语种英语
WOS记录号WOS:000342656100040
公开日期2014-11-21
源URL[http://159.226.149.42:8088/handle/152453/8173]  
专题昆明动物研究所_动物生态学研究中心
昆明动物研究所_遗传资源与进化国家重点实验室
作者单位1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
2.Kunming College of Life Sciences, University of Chinese Academy of Sciences, Kunming, Yunnan 650223, China
3.Department of Terrestrial Zoology, Nationaal Natuurhistorisch Museum Naturalis, 2300 RA Leiden, The Netherlands
4.Soil Science of Tropical and Subtropical Ecosystems, Büsgen Institute, Georg-August-Universität Göttingen, Büsgenweg 2, 37077 Göttingen, Germany
5.Key Laboratory for Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Yunnan 666303, China
6.School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, Norfolk NR4 7TJ, UK
推荐引用方式
GB/T 7714		 Yang CX,Wang XY,Miller JA,et al. Using metabarcoding to ask if easily collected soil and leaf-litter samples can be used as a general biodiversity indicator[J]. ECOLOGICAL INDICATORS,2014,46(X):379-389.
APA Yang CX.,Wang XY.,Miller JA.,de Blecourt M.,Ji YQ.,...&Yu DW[*].(2014).Using metabarcoding to ask if easily collected soil and leaf-litter samples can be used as a general biodiversity indicator.ECOLOGICAL INDICATORS,46(X),379-389.
MLA Yang CX,et al."Using metabarcoding to ask if easily collected soil and leaf-litter samples can be used as a general biodiversity indicator".ECOLOGICAL INDICATORS 46.X(2014):379-389.

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来源：昆明动物研究所

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