Temporal and Spatial Expression Patterns of Sox 1 Gene in Xenopus laevis Embryo
文献类型：期刊论文
| 作者 | Ma L1,2; Zhao SH1,2; Kong QH1,2; Mao BY[*]1 |
| 刊名 | 动物学研究
 |
| 出版日期 | 2007 |
| 卷号 | 28期号:4页码:403-408 |
| 关键词 | Sox1 Sox2 Sox3 Xenopus laevis Expression pattern |
| 其他题名 | Sox1基因在爪蟾早期发育中的时空表达图式 |
| 通讯作者 | mao@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 中文摘要 | 克隆了非洲爪蟾的Sox1基因并研究了它在非洲爪蟾早期发育过程中的时空表达图式,比较了Sox1-3基因在发育的脑和眼中的表达图式.序列比对分析显示Sox1-3蛋白在其HMG框结构域具有高度的保守性.通过RT-PCR方法分析了Sox1基因在爪蟾早期不同发育时段的表达情况,结果显示Sox1基因从未受精卵到尾芽期均有表达,但表达强度有所差异.原位杂交结果显示,在早期卵裂阶段和囊胚期,Sox1基因主要在动物极表达;从神经板期开始,Sox1基因主要在中枢神经系统和眼原基中表达.在蝌蚪期,Sox1与Sox2、Sox3在脑部和眼睛的表达区域有所不同.对于爪蟾Sox1基因时空表达图式的研究将有助于阐明SoxB1基因家族在脊椎动物神经系统发生过程中的作用. |
| 英文摘要 | We describe the temporal and spatial expression pattern of Sox 1 gene during Xenopus laevis early development and compare the expression patterns of Sox 1-3 in the developing eye and brain. Alignment of Sox 1-3 amino acid sequences shows a high conservation within the HMG-box DNA binding domains. RT-PCR analysis indicates that Sox 1 is expressed throughout development from the unfertilized egg to at least the tadpole stage, although at different expression levels. The transcripts of XSox 1 are detected in the animal pole at cleavage and blastrula stages and mainly in the central nervous system （CNS） and the developing eye at neurula stages. The study of the developmental expression of XSox 1 will aid in the elucidation of the function of SoxB 1 subgroup genes in vertebrate neurogenesis. |
| 收录类别 | 其他 |
| 资助信息 | 国家杰出青年科学基金(30425011);国家自然科学基金重点项目(3053038) |
| 语种 | 英语 |
| 公开日期 | 2010-08-06 |
| 源URL | [http://159.226.149.42:8088/handle/152453/995]  |
| 专题 | 昆明动物研究所_发育生物学 |
| 作者单位 | 1.CAS-Max Planck Junior Scientist Group on Neural Patterning, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China |
| 推荐引用方式 GB/T 7714 | Ma L,Zhao SH,Kong QH,et al. Temporal and Spatial Expression Patterns of Sox 1 Gene in Xenopus laevis Embryo[J]. 动物学研究,2007,28(4):403-408. |
| APA | Ma L,Zhao SH,Kong QH,&Mao BY[*].(2007).Temporal and Spatial Expression Patterns of Sox 1 Gene in Xenopus laevis Embryo.动物学研究,28(4),403-408. |
| MLA | Ma L,et al."Temporal and Spatial Expression Patterns of Sox 1 Gene in Xenopus laevis Embryo".动物学研究 28.4(2007):403-408. |
入库方式： OAI收割
来源：昆明动物研究所
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