Embryonic expression and evolutionary analysis of the amphioxus Dickkopf and Kremen family genes
文献类型：期刊论文
| 作者 | Zhang YJ1,2; Mao BY[*]1 |
| 刊名 | JOURNAL OF GENETICS AND GENOMICS
 |
| 出版日期 | 2010 |
| 卷号 | 37期号:9页码:637-645 |
| 关键词 | amphioxus Dickkopf Kremen evolution expression patter |
| 通讯作者 | mao@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | The secreted Wnt signaling inhibitor Dickkopf1 (Dkk1) plays key role in vertebrate head induction. Its receptor Kremen synergizes with Dkk1 in Wnt inhibition. Here we have carried out expression and functional studies of the Dkk and Kremen genes in amphioxus (Branchiostoma belcheri). During embryonic and larval development, BbDkk1/2/4 is expressed in the posterior mesoendoderm, anterior somatic mesoderm and the pharyngeal regions. Its expression becomes restricted to the pharyngeal region on the left side at larval stages. In 45 h larvae, BbDkk1/2/4 is expressed specifically in the cerebral vesicle. BbDkk3 was only detected at larval stages in the mid-intestine region. Seven Kremen related genes were identified in the genome of the Florida amphioxus (Branchiostoma floridae), clustered in 4 scaffolds, and are designated Kremen1-4 and Kremen-like 1-3, respectively. In B. belcheri, Kremen1 is strongly expressed in the mesoendoderm during early development and Kremen3 is expressed asymmetrically in spots in the larval pharyngeal region. In luciferase reporter assays, BbDkk1/2/4 can strongly inhibit Wnt signaling, while BbDkk3, BbKremen1 and BbKremen3 can not. No co-operative effect was observed between amphioxus Dkk1/2/4 and Kremens, suggesting that the interaction between Dkk and Kremen likely originated later during evolution |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the grant from the Innova- tion Project of Chinese Academy of Sciences (No. KSCX2-YW-R-090). We thank Prof. Junyuan Chen (Nan- jing University) for amphioxus materials. |
| 语种 | 英语 |
| 公开日期 | 2010-11-01 |
| 源URL | [http://159.226.149.42:8088/handle/152453/6345]  |
| 专题 | 昆明动物研究所_发育生物学 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
| 推荐引用方式 GB/T 7714 | Zhang YJ,Mao BY[*]. Embryonic expression and evolutionary analysis of the amphioxus Dickkopf and Kremen family genes[J]. JOURNAL OF GENETICS AND GENOMICS,2010,37(9):637-645. |
| APA | Zhang YJ,&Mao BY[*].(2010).Embryonic expression and evolutionary analysis of the amphioxus Dickkopf and Kremen family genes.JOURNAL OF GENETICS AND GENOMICS,37(9),637-645. |
| MLA | Zhang YJ,et al."Embryonic expression and evolutionary analysis of the amphioxus Dickkopf and Kremen family genes".JOURNAL OF GENETICS AND GENOMICS 37.9(2010):637-645. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。