Henryin, an ent-kaurane Diterpenoid, Inhibits Wnt Signaling through Interference with beta-Catenin/TCF4 Interaction in Colorectal Cancer Cells
文献类型：期刊论文
| 作者 | Li XY1,3; Pu JX1; Jiang SY2,3; Su J1,3; Kong LM1,3; Mao BY2; Sun HD[*]1; Li Y[*]1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:7页码:e68525 |
| 通讯作者 | hdsun@mail.kib.ac.cn ; liyanb@mail.kib.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Aberrant Wnt/beta-catenin signaling has been strongly associated with the tumorigenesis of human colorectal cancer. Inhibitors of this pathway may then offer therapeutic strategies as well as chemoprevention for this malignant disease. Henryin is an ent-kaurane diterpenoid isolated from Isodon rubescens var. lushanensis, a plant long been used in folk medicine to prevent inflammation and gastrointestinal disease. In the present study, we report that henryin selectively inhibits the proliferation of human colorectal cancer cells with a GI(50) value in the nano-molar range. Microarray analysis and reporter assays showed that henryin worked as a novel antagonist of Wnt signaling pathway. Henryin reduced the expression of Cyclin D1 and C-myc, and induced G1/S phase arrest in HCT116 cells. Concurrently, henryin did not affect the cytosol-nuclear distribution of soluble beta-catenin, but impaired the association of beta-catenin/TCF4 transcriptional complex likely through directly blocking the binding of beta-catenin to TCF4. We also then analyzed the structure-activity relationship among the ent-kaurane type diterpenoids. Our data suggests that henryin, as a novel inhibitor of Wnt signaling, could be a potential candidate for further preclinical evaluation for colon cancer treatment, and as such warrants further exploration. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by both the 100 Talents Program (YL) and the West Light Foundation (JP) of the Chinese Academy of Sciences, the Major State Basic Research Development Program of China (No. 2009CB522300), the Natural Science Foundation of China (No.81173076, 81172939), and the Recruited Top Talent of Sciences and Technology of Yunnan Province (2009C1120), China. |
| 语种 | 英语 |
| WOS记录号 | WOS:000321341000169 |
| 公开日期 | 2014-10-17 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8085]  |
| 专题 | 昆明动物研究所_发育生物学 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China 2.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 3.University of the Chinese Academy of Sciences, Beijing, China |
| 推荐引用方式 GB/T 7714 | Li XY,Pu JX,Jiang SY,et al. Henryin, an ent-kaurane Diterpenoid, Inhibits Wnt Signaling through Interference with beta-Catenin/TCF4 Interaction in Colorectal Cancer Cells[J]. PLOS ONE,2013,8(7):e68525. |
| APA | Li XY.,Pu JX.,Jiang SY.,Su J.,Kong LM.,...&Li Y[*].(2013).Henryin, an ent-kaurane Diterpenoid, Inhibits Wnt Signaling through Interference with beta-Catenin/TCF4 Interaction in Colorectal Cancer Cells.PLOS ONE,8(7),e68525. |
| MLA | Li XY,et al."Henryin, an ent-kaurane Diterpenoid, Inhibits Wnt Signaling through Interference with beta-Catenin/TCF4 Interaction in Colorectal Cancer Cells".PLOS ONE 8.7(2013):e68525. |
入库方式： OAI收割
来源：昆明动物研究所
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