Frequent loss of heterozygosity on chromosome 12q in non-small-cell lung carcinomas
文献类型：期刊论文
| 作者 | Ding XJ1; Liu MX1,2; Ao L1; Liang YR1; Cao Y[*]1 |
| 刊名 | VIRCHOWS ARCHIV
 |
| 出版日期 | 2011 |
| 卷号 | 458期号:5页码:561-569 |
| 关键词 | Non-small-cell lung carcinoma (NSCLC) Chromosome Karyotype 12q Loss of heterozygosity |
| 通讯作者 | caoy@mail.kiz.ac.cn |
| 英文摘要 | Chromosomal aberrations in non-small-cell lung carcinomas (NSCLCs) are common events. In our study, the lung cancer cell lines (NCI-H446 and SPC-A-1) displayed numerous numerical and structural alterations in their chromosomes by G-banded karyotypic analysis, and abnormalities of chromosome 12 by fluorescence in situ hybridization. Sequentially, we used 14 microsatellite markers within 12q to analyze loss of heterozygosity (LOH) in lung cancer cell lines and NSCLCs. Possible LOH on 12q were statistically inferred to occur in five lung cell lines. Importantly, 17 out of 25 NSCLCs (68%) showed LOH at chromosome 12q. Frequencies of LOH for individual markers ranged from 18% to 44%. Several deletions which were marked with D12S1301, D12S2196, D12S398, D12S90, D12S1056, D12S1713, D12S375, D12S1040, D12S326, and D12S106 were newly detected. Allelic loss on 12q15-q21 detected with D12S1040 occurred at the later stages of NSCLC progression (p<0.05, Fisher's exact test). LOH on 12q marked with D12S2196, D12S398, D12S326, and D12S106 were frequently found in NSCLCs from the patients without smoking history (p<0.05, Fisher's exact test). These findings indicated that allelic loss on 12q is commonly involved in NSCLCs, and new tumor suppressor genes may occur within 12q. |
| 资助信息 | Supported by Hundred Talents Program (Grant Cao) and West Light Foundation (Grant Liang) of Chinese Academy of Sciences. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2011-06-13 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6625]  |
| 专题 | 昆明动物研究所_分子病理学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of CAS and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, Yunnan 650223, China 2.Graduate University of the Chinese Academy of Sciences, Beijing 100049, China |
| 推荐引用方式 GB/T 7714 | Ding XJ,Liu MX,Ao L,et al. Frequent loss of heterozygosity on chromosome 12q in non-small-cell lung carcinomas[J]. VIRCHOWS ARCHIV,2011,458(5):561-569. |
| APA | Ding XJ,Liu MX,Ao L,Liang YR,&Cao Y[*].(2011).Frequent loss of heterozygosity on chromosome 12q in non-small-cell lung carcinomas.VIRCHOWS ARCHIV,458(5),561-569. |
| MLA | Ding XJ,et al."Frequent loss of heterozygosity on chromosome 12q in non-small-cell lung carcinomas".VIRCHOWS ARCHIV 458.5(2011):561-569. |
入库方式： OAI收割
来源：昆明动物研究所
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