ERGIC3, which is regulated by miR-203a, is a potential biomarker for non-small cell lung cancer
文献类型：期刊论文
| 作者 | Lin QH1; Zhang KD1; Cao Y[*]1; Duan HX1; Liu MX1; Wei WL2 |
| 刊名 | CANCER SCIENCE
 |
| 出版日期 | 2015 |
| 卷号 | 106期号:10页码:1463-1473 |
| 关键词 | Biomarker ERGIC3 miR-203a monoclonal antibody non-small cell lung cancer |
| 通讯作者 | caoy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | In a previous study, we found that ERGIC3 was a novel lung cancer-related gene by screening libraries of differentially expressed genes. In this study, we developed a new murine monoclonal antibody (mAb) against ERGIC3. This avid antibody (6-C4) is well suited for immunohistochemistry, immunoblotting and solid-phase immunoassays. Furthermore, we systematically investigated expressions of ERGIC3 in a broad variety of normal human tissues and various types of tumors by immunohistochemistry. In normal human tissues, 6-C4 reacted only in some epithelial cells, such as hepatocytes, gastrointestinal epithelium, ducts and acini of the pancreas, proximal and distal tubules of the kidney, and mammary epithelial cells; however, most normal human tissues were not stained. Moreover, almost all carcinomas that originated from the epithelial cells were positive for 6-C4, whereas all sarcomas were negative. Notably, 6-C4 strongly stained non-small cell lung cancer (NSCLC) cells but did not react with normal lung tissues. Hence, ERGIC3 mAb could be used in histopathological diagnosis and cytopathological testing to detect early-stage NSCLC. We also studied the mechanisms of ERGIC3 regulation in vitro and in vivo by means of bioinformatics analysis, luciferase reporter assay, miRNA expression profiling and miRNA transfection. Results showed that miR-203a downregulation induced ERGIC3 overexpression in NSCLC cells. |
| 收录类别 | SCI |
| 资助信息 | Natural Science Foundation of China (81272617) and the 973 Program (2011CB510104). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9378]  |
| 专题 | 昆明动物研究所_分子病理学 |
| 作者单位 | 1.Laboratory of Molecular and Experimental Pathology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 2.Department of Pathology, Yunnan Tumor Hospital, Kunming, China |
| 推荐引用方式 GB/T 7714 | Lin QH,Zhang KD,Cao Y[*],et al. ERGIC3, which is regulated by miR-203a, is a potential biomarker for non-small cell lung cancer[J]. CANCER SCIENCE,2015,106(10):1463-1473. |
| APA | Lin QH,Zhang KD,Cao Y[*],Duan HX,Liu MX,&Wei WL.(2015).ERGIC3, which is regulated by miR-203a, is a potential biomarker for non-small cell lung cancer.CANCER SCIENCE,106(10),1463-1473. |
| MLA | Lin QH,et al."ERGIC3, which is regulated by miR-203a, is a potential biomarker for non-small cell lung cancer".CANCER SCIENCE 106.10(2015):1463-1473. |
入库方式： OAI收割
来源：昆明动物研究所
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