CD176 single-chain variable antibody fragment inhibits the adhesion of cancer cells to endothelial cells and hepatocytes
文献类型：期刊论文
| 作者 | Liu JN1,2; Yi B1,2; Zhang Z1,2; Cao Y[*]1 |
| 刊名 | FRONTIERS OF MEDICINE
 |
| 出版日期 | 2016 |
| 卷号 | 10期号:2页码:204-211 |
| 关键词 | CD176 Thomsen-Friedenreich antigen scFv cancer therapy adhesion metastasis |
| 通讯作者 | caoy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | CD176 (Thomsen-Friedenreich antigen) is a tumor-associated carbohydrate epitope (glycotope) functionally involved in blood spread and liver metastasis of cancer cells by mediating the adhesion of cancer cells to endothelial cells and hepatocytes, respectively. CD176 could be a promising target for antitumor immunotherapy. We applied B lymphocytes obtained from mice immunized with CD176 antigen and constructed a phage display library. A positive clone of CD176 single-chain variable antibody fragment (scFv) was successfully screened from this library. TheCD176 scFv was expressed in Escherichia coli and purified. The purified scFv can bind to the natural CD176 expressed on the surface of cancer cells. Furthermore, the CD176 scFv inhibits the adhesion of CD176(+) cancer cells to endothelial cells and hepatocytes. This CD176 scFv provides a basis for future development of recombinant CD176-specific antibodies that can be used in therapeutic application. |
| 收录类别 | 其他 |
| 资助信息 | This work was supported by National Natural Science Foundation of China (No. 81072563) and the Chinese Academy of Sciences (KSCX2-YW-R-196). We especially thank Dr. Reinhard Schwartz- Albiez (German Cancer Research Center, Heidelberg, Germany) for his support and valuable critical comments. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10240]  |
| 专题 | 昆明动物研究所_分子病理学 |
| 作者单位 | 1.Laboratory of Molecular and Experimental Pathology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming 650223, China |
| 推荐引用方式 GB/T 7714 | Liu JN,Yi B,Zhang Z,et al. CD176 single-chain variable antibody fragment inhibits the adhesion of cancer cells to endothelial cells and hepatocytes[J]. FRONTIERS OF MEDICINE,2016,10(2):204-211. |
| APA | Liu JN,Yi B,Zhang Z,&Cao Y[*].(2016).CD176 single-chain variable antibody fragment inhibits the adhesion of cancer cells to endothelial cells and hepatocytes.FRONTIERS OF MEDICINE,10(2),204-211. |
| MLA | Liu JN,et al."CD176 single-chain variable antibody fragment inhibits the adhesion of cancer cells to endothelial cells and hepatocytes".FRONTIERS OF MEDICINE 10.2(2016):204-211. |
入库方式： OAI收割
来源：昆明动物研究所
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