MicroRNA-944 Affects Cell Growth by Targeting EPHA7 in Non-Small Cell Lung Cancer
文献类型：期刊论文
| 作者 | Liu MX1; Zhou KC1,2; Cao Y[*]1 |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2016 |
| 卷号 | 17期号:10页码:e1493 |
| 关键词 | EPHA7 cell proliferation miR-944 non-small cell lung cancer (NSCLC) regulation |
| 通讯作者 | caoy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | MicroRNAs (miRNAs) have critical roles in lung tumorigenesis and development. To determine aberrantly expressed miRNAs involved in non-small cell lung cancer (NSCLC) and investigate pathophysiological functions and mechanisms, we firstly carried out small RNA deep sequencing in NSCLC cell lines (EPLC-32M1, A549 and 801D) and a human immortalized cell line 16HBE, we then studied miRNA function by cell proliferation and apoptosis. cDNA microarray, luciferase reporter assay and miRNA transfection were used to investigate interaction between the miRNA and target gene. miR-944 was significantly down-regulated in NSCLC and had many putative targets. Moreover, the forced expression of miR-944 significantly inhibited the proliferation of NSCLC cells in vitro. By integrating mRNA expression data and miR-944-target prediction, we disclosed that EPHA7 was a potential target of miR-944, which was further verified by luciferase reporter assay and microRNA transfection. Our data indicated that miR-944 targets EPHA7 in NSCLC and regulates NSCLC cell proliferation, which may offer a new mechanism underlying the development and progression of NSCLC. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the Natural Science Foundation of China (81272617), the 973 Program (2011CB510104), and the Yunnan Province Science and Technology Department (Y103951111). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10556]  |
| 专题 | 昆明动物研究所_分子病理学 |
| 作者单位 | 1.Laboratory of Molecular and Experimental Pathology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming 650223, China |
| 推荐引用方式 GB/T 7714 | Liu MX,Zhou KC,Cao Y[*]. MicroRNA-944 Affects Cell Growth by Targeting EPHA7 in Non-Small Cell Lung Cancer[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(10):e1493. |
| APA | Liu MX,Zhou KC,&Cao Y[*].(2016).MicroRNA-944 Affects Cell Growth by Targeting EPHA7 in Non-Small Cell Lung Cancer.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(10),e1493. |
| MLA | Liu MX,et al."MicroRNA-944 Affects Cell Growth by Targeting EPHA7 in Non-Small Cell Lung Cancer".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.10(2016):e1493. |
入库方式： OAI收割
来源：昆明动物研究所
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