中国部分猪种SLA2DQB 外显子2 遗传多样性
文献类型：期刊论文
| 作者 | 李华1,2,3; 张亚平[*]1; 邱祥聘3 |
| 刊名 | 遗传
 |
| 出版日期 | 2005 |
| 卷号 | 27期号:2页码:173～180 |
| 关键词 | 中国猪种 SLA2DQB 遗传多样性 |
| 其他题名 | The Genetic Diversity of the Second Exon About SLA-DQB in Some Chinese Native Pigs |
| 通讯作者 | zhangyp@public.km.yn.cn |
| 合作状况 | 其它 |
| 中文摘要 | 运用PCR2SSCP 和克隆测序对中国部分猪种的SLA2DQB 基因外显子2 的多态性分析表明:有功能的 DQB 基因有68 个新等位基因,假基因(SLA2DXB) 等位基因有5 个。各等位基因的数量分布极其不平衡,而且许多 品种都表现出共享等位基因。9 个主要等位基因中, C08 广泛分布在中国猪种的6 大地方类型11 个品种及云南 和四川野猪中,它为中国猪种特有的共享等位基因,占总数的55. 10 %。在单一的个体中拥有5 条以上序列,说明 SLA2DQB 基因座在某些品种中拷贝数为3 个。SLA2DQB 外显子2 的等位基因核苷酸和氨基酸多态变异位点分别 高达81 个和49 个,等位基因多样度( H= 0. 889) 以及核苷酸多样度( Pi = 0. 047) 都很高,总体表现为β折叠区的Pi 值均高于α螺旋区。综合分析表明华南型、西南型猪H和Pi 均较高,高原型的藏猪最低。类群内遗传距离排序与 Pi 值高低排序一样,可见各地方类型中核苷酸替换的差异正比于核苷酸多样度。类群间遗传距离比较,江海型与 华北型间的距离最大,而华中型和高原型间的遗传距离最小。 |
| 英文摘要 | Polymorphism of the second exon about SLA-DQB genes in some Chinese native pigs was investigated employing PCR–SSCP analysis and DNA cloning sequencing. Combined with the sequences in GenBank, 73 new alleles were gained, among them function alleles of SLA-DQB were sixty-eight, five alleles of pseudogene (SLA-DXB) were gained. There were disequilibrium distribution of the alleles in all breeds, the same allele was shared by most breeds. C08 allele existed in 11 breeds of six groups and in the wild pigs of Yunnan and Sichuan province, and its frequency reached 55.10%. More than five sequences were found in each individual, suggesting duplication in these loci examined, there were three copies about SLA-DQB genes. The variable sites based on nucleotide and amino acids reached to eighty-one and forty-nine, respectively. The values of allelic diversity (H=0.889) and the nucleotide diversity (Pi=0.047) were revealed very high, from both values of H and Pi were higher in the β pleated sheet region than those in α helix region in Chinese native pigs. In general, values of H and Pi in Huanan pigs and Xinan pigs were higher, vice versa in Tibet pigs. The genetic distance within group were showed the same order as comparison Pi values, hence we concluded the different rate of nucleotide substitution were positive correlated with the degree of nucleotide diversity in Chinese domestic groups. The genetic distances about SLA-DQB gene between groups were detected as follow: the great genetic distance were existed between the Jianghai and Huabei groups, and the smallest genetic distance were existed between the Huazhong and Gaoyuan groups. |
| 收录类别 | 其他 |
| 资助信息 | 国家重点基础研究发展规划项目资助 ( 编号G20000161) |
| 语种 | 中文 |
| 公开日期 | 2010-08-10 |
| 源URL | [http://159.226.149.42:8088/handle/152453/1199]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_细胞与分子进化重点实验室 |
| 作者单位 | 1.中国科学院昆明动物研究所细胞与分子进化重点实验室 , 昆明 650223 2.佛山科学技术学院动物科学系;佛山 528200 3.四川农业大学动物科技学院;雅安 625014 |
| 推荐引用方式 GB/T 7714 | 李华,张亚平[*],邱祥聘. 中国部分猪种SLA2DQB 外显子2 遗传多样性[J]. 遗传,2005,27(2):173～180. |
| APA | 李华,张亚平[*],&邱祥聘.(2005).中国部分猪种SLA2DQB 外显子2 遗传多样性.遗传,27(2),173～180. |
| MLA | 李华,et al."中国部分猪种SLA2DQB 外显子2 遗传多样性".遗传 27.2(2005):173～180. |
入库方式： OAI收割
来源：昆明动物研究所
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