RAPD分析与白头叶猴分类地位探讨
文献类型:期刊论文
作者 | 丁波1; 张亚平[*]1; 刘自明2; 韦毅2 |
刊名 | 动物学研究
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出版日期 | 1999 |
卷号 | 20期号:1页码:1-6 |
关键词 | 黑叶猴 白头叶猴 亚种 随机扩增多态DNA(RAPD) |
ISSN号 | 0254-5853 |
其他题名 | Preliminary Report on Classification Status of The White-Headed Langur Inferred from Random Amiplified Polymorphism DNA |
中文摘要 | 根据遗传距离建立的系统树显示,黑叶猴与白头叶猴亲缘关系最近,且两者都不是单系群。白头叶猴3与黑叶猴6的亲缘关系比与白头叶猴1、2的更近。根据系统树中的关系对群体进行了t检验,结果显示:在5%水平上,白头叶猴与黑叶猴有显著差异;然而,在同样的水平上白头叶猴1、2、3和黑叶猴6、2、3组成的群体也显著区别于黑叶猴1、4、5组成的群体。系统树和t检验结果表明,白头叶猴与黑叶猴之间遗传差异水平较低,近期两者之间可能存在基因流。结合形态学特征和地理分布特点,结果支持白头叶猴可能是黑叶猴的一个亚种的观点。 |
英文摘要 | The classification of the white-headed langur has remained controversial.Tan (1957) proposed that the white-headed langur,discovered by Tan in 1952 in southwestern Guangxi Autonomous Region,be a new species Presbytis leucocephalus.Li and Ma (1980) recognized the white-headed langur as a subspecies of P.francoisi based on a field survey of the geographical distribution of both the Francois langur and the white-headed langur in southern Guangxi Autonomous Region.Lu and Huang (1993) found that altitude coincides with habitat use,and separates Francois langur from white-headed langurs.So they distinguished the langur as separate species.All of viewpoints mentioned above are mainly based on gross morphological features and geographical distribution.Random amplified polymorphism DNA (RAPD) of 2 T.phayrei,1 T.vetulus,1 Semnopithecus entellus and 6 T.francoisi,and 3 white-headed langurs were analyzed in order to clarify the classification status of the whiteheaded langur.A total of 30 10-mer arbitrary primers were used and 22 of which generate clear bands.The phylogenetic tree constructed on the basis of genetic distance shows that the whole tree should be sorted into two clusters:(1)T.phayrei and (2)T.f.leucocephalus and T.francoisi.Our results show that T.francoisi and T.f.leucocephalus are more closely related to each other than to T.phayrei.The T.francoisi and T.f.leucocephalus are not monophyletic groups.However,T.francoisi.2,3,6 are more closely related to T.f.leucocephalus 1,2,3 than to T.francoisi 1,4,5.Students s test was performed among groups based on the phylogenetic tree.The statistic tests show that T.f.leucocephalus is different from T.francoisi at 5% significant level and the group T.f.leucocephalus 1,2,3,T.francoisi.2,3,6 is different from group T.francoisi1,4,5 at 5% significant level.The other three groups are not different from each other at 5% significant level.Our phylogenetic tree and students test results suggest that a genetic differentiation exists in T.francoisi and T.f.leucocephalus at a low level.Considering the morphological features and geographical distribution,we think that the whiteheaded langur might be a subspecies.A gene flow may exist between the white-headed langur and Francois langur in recent years. |
收录类别 | 其他 |
资助信息 | 国家自然科学基金,云南省自然科学基金,中国科学院基金 |
原文出处 | 19992011.pdf |
语种 | 中文 |
公开日期 | 2010-08-24 |
源URL | [http://159.226.149.42:8088/handle/152453/2281] ![]() |
专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_细胞与分子进化开放实验室 |
作者单位 | 1.中国科学院昆明动物研究所细胞与分子进化开放研究实验室 2.林业部广西野生动物与自然保护管理站 |
推荐引用方式 GB/T 7714 | 丁波,张亚平[*],刘自明,等. RAPD分析与白头叶猴分类地位探讨[J]. 动物学研究,1999,20(1):1-6. |
APA | 丁波,张亚平[*],刘自明,&韦毅.(1999).RAPD分析与白头叶猴分类地位探讨.动物学研究,20(1),1-6. |
MLA | 丁波,et al."RAPD分析与白头叶猴分类地位探讨".动物学研究 20.1(1999):1-6. |
入库方式: OAI收割
来源:昆明动物研究所
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