家养双峰驼线粒体DNA遗传多样性的研究
文献类型：期刊论文
| 作者 | 权洁霞1,2; 张亚平[*]1; 韩建林2; 门正明2 |
| 刊名 | 遗传学报
 |
| 出版日期 | 2000 |
| 卷号 | 27期号:5页码:383-390 |
| 关键词 | 双峰驼 线粒体DNA 遗传多样性 限制性片段长度多态性 |
| ISSN号 | 0379-4172 |
| 通讯作者 | zhangyp@public.km.yn.cn |
| 中文摘要 | 在双峰驼mtDNA中未发现长度变异和个体内异质现象。从3个类群的87个个体中，共检出了35种限制性态型，12种mtDNA单倍型，家驼群内核苷酸歧异度（#pi#）为0.2273%，这表明我国的家驼在mtDNA水平存在较丰富的遗传多样性；单倍型分布的差异显著性检验及单倍型的共享度分析表明，mtDNA在这3个类群之间没有明显的遗传分化，其较丰富的遗传变异主要来自群体内的差异，与类群间的遗传分化关系不大。 |
| 英文摘要 | In this paper the mitochondrial DNA (mtDNA) of 87 domestic camels from Gansu, Xingjiang and Inner Mongolia were analyzed. Totally 18 restriction endonucleases including Apa I, Ava I, BamH I, Bcl I, Bgl I, Bgl II, Dra I, EcoR I, EcoR V, Hind III, Kpn I, Pvu II, Sac I, Sal I, Sca I, Sma I, Xba I and Xho I were used to investigate the restriction fragment length polymorphism (RFLP) of camel mtDNA through Southern blotting to reveal the genetic diversity within three groups of domestic camel and the genetic relationships among the three domestic groups. The results indicated that the average molecular weight of camel mtDNA is 16.26 +/- 0.28 kb, the camel mtDNA is inherited maternally and no variations and heteroplasmy are found in camel mtDNA. Among the 82 domestic camels, 35 restriction morph and 12 haplotypes were detected, and the value of average sequence variation within the domestic population (pi) is 0.2273%, which showed a relatively rich genetic diversity existed in the domestic camels. From F-test of the haplotype distribution and comparison of the haplotype sharement between the 3 domestic camel groups, it was suggested that there is no obvious genetic divergence and that the rich genetic diversity comes from the variation among individuals instead of the genetic divergence between groups. All domestic camels could be divided into 2 types (A and B) with a significant genetic distance (P = 0.0464). |
| 收录类别 | 其他 |
| 资助信息 | 中科院昆明动物研究所细胞与分子进化开放研究实验室开放课题 |
| 原文出处 | 2000275383.pdf |
| 语种 | 中文 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2311]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_细胞与分子进化开放实验室 |
| 作者单位 | 1.中国科学院昆明动物研究所细胞与分子进化开放实验室;昆明650223 2.甘肃农业大学动物生产工程系;兰州730070 |
| 推荐引用方式 GB/T 7714 | 权洁霞,张亚平[*],韩建林,等. 家养双峰驼线粒体DNA遗传多样性的研究[J]. 遗传学报,2000,27(5):383-390. |
| APA | 权洁霞,张亚平[*],韩建林,&门正明.(2000).家养双峰驼线粒体DNA遗传多样性的研究.遗传学报,27(5),383-390. |
| MLA | 权洁霞,et al."家养双峰驼线粒体DNA遗传多样性的研究".遗传学报 27.5(2000):383-390. |
入库方式： OAI收割
来源：昆明动物研究所
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