The giant panda (Ailurapoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg
文献类型：期刊论文
| 作者 | Chen DY1; Sun QY1; Liu JL1; Li GP1; Lian L1; Wang MK1; Han ZM1; Song XF1; Li JS1; Sun Q1 |
| 刊名 | SCIENCE IN CHINA SERIES C-LIFE SCIENCES
 |
| 出版日期 | 1999 |
| 卷号 | 42期号:4页码:346-353 |
| 关键词 | giant panda rabbit interspecies cloning dedifferentiation totipotency |
| ISSN号 | 1006-9305 |
| 产权排序 | The giant panda (Ailurapoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg |
| 合作状况 | 其它 |
| 英文摘要 | The giant panda skeletal muscle cells, uterus epithelial cells and mammary gland cells from an adult individual were cultured and used as nucleus donor for the construction of interspecies embryos by transferring them into enucleated rabbit eggs. All the three kinds of somatic cells were able to reprogram in rabbit ooplasm and support early embryo development, of which mammary gland cells were proven to be the Lest, followed by uterus epithelial cells and skeletal muscle cells. The experiments showed that direct injection of mammary gland cell into enucleated rabbit ooplasm, combined with in vivo development in ligated rabbit oviduct, achieved higher blastocyst development than in vitro culture after the somatic cell was injected into the perivitelline space and fused with the enucleated egg by electrical stimulation. The chromosome analysis demonstrated that the genetic materials in reconstructed blastocyst cells were the same as that in panda somatic cells. In addition, giant panda mitochondrial DNA (mtDNA) was shown to exist in the interspecies reconstructed blastocyst. The data suggest that (i) the ability of ooplasm to dedifferentiate somatic cells is not species-specific; (ii) there is compatibility between interspecies somatic nucleus and ooplasm during early development of the reconstructed egg. |
| 收录类别 | SCI |
| 原文出处 | 199942346.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/3915]  |
| 专题 | 昆明动物研究所_分子进化基因组学 |
| 作者单位 | 1.State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China 2.Fuzhou Giant Panda Research Center, Fuzhou 350001, China 3.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China |
| 推荐引用方式 GB/T 7714 | Chen DY,Sun QY,Liu JL,et al. The giant panda (Ailurapoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,1999,42(4):346-353. |
| APA | Chen DY.,Sun QY.,Liu JL.,Li GP.,Lian L.,...&Ding B.(1999).The giant panda (Ailurapoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,42(4),346-353. |
| MLA | Chen DY,et al."The giant panda (Ailurapoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 42.4(1999):346-353. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。