Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda
文献类型：期刊论文
| 作者 | Ding B1; Shi P1; Xiangyu JG1; Zhang YP[*]1; Chen DY2; Sun QY2; Li GP2; Wang MK2; Liu YL2; Kang L2 |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2000 |
| 卷号 | 45期号:20页码:1883-1885 |
| 关键词 | giant panda (Ailuropoda melanoleuca) interspecies cloning microsatellite DNA nucleus |
| ISSN号 | 1001-6538 |
| 通讯作者 | zhangyp@public.km.yn.cn |
| 合作状况 | 其它 |
| 英文摘要 | A method for DNA isolation from early development of blastocyst and further analysis of nuclear and mitochondrial DNA was developed in present study. Total DNA was prepared from interspecies reconstructed blastocyst and a giant panda specific microsatellite locus g(010) was successfully amplified. DNA sequencing of the PCR product showed that two sequences of reconstructed blastocysts are the same as that of positive control giant panda. Our results prove that the nucleus of interspecies reconstructed blastocyst comes from somatic nucleus of donor giant panda. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the 1998 Special Fund of the Chinese Academy of Sciences (Grant No. KY95-JI-318) and the 1999 Special Fund from the Ministry of Science and Technology of China |
| 原文出处 | 2000451883.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/3963]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_进化与功能基因组学 昆明动物研究所_细胞与分子进化开放实验室 |
| 作者单位 | 1.Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences. Kunming 650223, China 2.State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China 3.Fuzhou Panda Institute Center, Fuzhou 350001, China |
| 推荐引用方式 GB/T 7714 | Ding B,Shi P,Xiangyu JG,et al. Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda[J]. CHINESE SCIENCE BULLETIN,2000,45(20):1883-1885. |
| APA | Ding B.,Shi P.,Xiangyu JG.,Zhang YP[*].,Chen DY.,...&Chen YC.(2000).Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda.CHINESE SCIENCE BULLETIN,45(20),1883-1885. |
| MLA | Ding B,et al."Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda".CHINESE SCIENCE BULLETIN 45.20(2000):1883-1885. |
入库方式： OAI收割
来源：昆明动物研究所
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