Characterization of Sarcocystis species in domestic animals using a PCR-RFLP analysis of variation in the 18S rRNA gene: a cost-effective and simple technique for routine species identification
文献类型：期刊论文
| 作者 | Yang ZQ1,2,3; Li QQ3; Zuo YX3; Chen XW3; Chen YJ1; Nie L1 ; Wei CG2; Zen JS2; Attwood SW4; Zhang XZ2
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| 刊名 | EXPERIMENTAL PARASITOLOGY
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| 出版日期 | 2002 |
| 卷号 | 102期号:3-4页码:212-217 |
| 关键词 | 18S ribosomal RNA (rRNA) gene small subunit (SSU) polymerase chain reaction (PCR)-restriction fragment length poly- morphism (RFLP) |
| ISSN号 | 0014-4894 |
| 通讯作者 | zhangyp@public.km.yn.cn |
| 合作状况 | 其它 |
| 英文摘要 | Thirteen restriction endonucleases were used to investigate nuclotide sequence variation in the 18S rRNA DNA of 88 individuals from ten Sarcocystis taxa collected as cysts from their intermediate hosts, swine, cattle and water buffalo. A DNA sequence of approximately 900 bp was used. A total of 26 electromorphs were detected. The electromorphs were sorted into seven different haplotypes that coincided with the six named species and an unidentified species from cattle. These findings support those of our morphological examinations, which suggested that the taxa resembling Sarcocystis hirsuta, S. hominis, both found in water buffalo, and S. sinensis found in cattle, are not new species but are in fact S. hirsuta and S. hominis as found in cattle, and S. sinensis as found in water buffalo; this finding supports the idea that these species can utilize both cattle and water buffalo as intermediate hosts and are not restricted to one or the other host group as previously thought. PCR-RFLP resolved by agarose gel electrophoresis is shown to be an easy and rapid method of discriminating between these species. |
| 收录类别 | SCI |
| 资助信息 | This study was funded by The Yunnan teaching committee foundation for science (No. 0011001); The foundation of Kunming Medical College for science, for advanced academic teacher and the College foundation for the PhD Pro-gram. The foundation for science of Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences. Xin-Wen Chen is supported by the Nature Science Foundation of China (No. 30160013). Dr. Attwood is supported by Welcome Project Grant No. 058932. |
| 原文出处 | 2002102212.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/4033]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_细胞与分子进化开放实验室 管理、支撑部门_信息网络中心 |
| 作者单位 | 1.Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Parasitology Department, Kunming Medical College, Kunming, Yunnan 650031, China 3.Biology Department, Yunnan University, Kunming, Yunnan 650091, China 4.Department of Zoology, The Natural History Museum, Cromwell Road, SW7 5BD London, UK |
| 推荐引用方式 GB/T 7714 | Yang ZQ,Li QQ,Zuo YX,et al. Characterization of Sarcocystis species in domestic animals using a PCR-RFLP analysis of variation in the 18S rRNA gene: a cost-effective and simple technique for routine species identification[J]. EXPERIMENTAL PARASITOLOGY,2002,102(3-4):212-217. |
| APA | Yang ZQ.,Li QQ.,Zuo YX.,Chen XW.,Chen YJ.,...&Zhang YP[*].(2002).Characterization of Sarcocystis species in domestic animals using a PCR-RFLP analysis of variation in the 18S rRNA gene: a cost-effective and simple technique for routine species identification.EXPERIMENTAL PARASITOLOGY,102(3-4),212-217. |
| MLA | Yang ZQ,et al."Characterization of Sarcocystis species in domestic animals using a PCR-RFLP analysis of variation in the 18S rRNA gene: a cost-effective and simple technique for routine species identification".EXPERIMENTAL PARASITOLOGY 102.3-4(2002):212-217. |
入库方式： OAI收割
来源：昆明动物研究所
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