A PCR-based RFLP analysis of Sarcocystis cruzi (Protozoa : Sarcocystidae) in Yunnan province, PR china, reveals the water buffalo (Bubalus bubalis) as a natural intermediate host
文献类型：期刊论文
| 作者 | Li QQ2,3; Yang ZQ[*]2; Zuo YX1; Attwood SW4; Chen XW1; Zhang YP[*]3 |
| 刊名 | JOURNAL OF PARASITOLOGY
 |
| 出版日期 | 2002 |
| 卷号 | 88期号:6页码:1259-1261 |
| ISSN号 | 0022-3395 |
| 通讯作者 | yzhaoqing@km169.net |
| 合作状况 | 其它 |
| 英文摘要 | A polymerase chain reaction-based restriction fragment length polymorphism (RFLP) approach is used to examine Sarcocystis cruzi-like taxa from the atypical intermediate host, water buffalo, in Yunnan, People's Republic of China. The loci examined lie within the 18S rRNA gene. A total of 15 water buffalo isolates are compared with those of 10 S. cruzi from cattle. RFLP patterns for the S. cruzi isolates from cattle and the S. cruzi-like taxon from water buffalo are found to be identical with all the 12 restriction enzymes used. Interpopulation variation between samples from Kunming and Gengma (Yunnan) is found to be undetectable at these loci for both S. cruzi and the S. cruzilike taxon. But RFLPs are found between the S. cruzi taxa and S. suihominis from pigs at the same study sites. These findings support the hypothesis that S. cruzi is able to use the water buffalo as an intermediate host and is not restricted to cattle as was previously supposed. |
| 收录类别 | SCI |
| 资助信息 | This study was funded by the Yunnan Teach- ing Committee Foundation for Science (grant 0011001), the Foundation for Science of Kunming Medical College, and the College Foundation for the Ph.D. program. Y.-P.Z. is supported by NSFC; X.-W.C. is sup- ported by the Natural Sciences Foundation of China (grant 30160013); S.W.A. is supported by Wellcome Trust Project (grant 058932). |
| 原文出处 | 2002881259.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/4039]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_细胞与分子进化开放实验室 |
| 作者单位 | 1.Biology Department, Yunnan University, Kunming, Yunnan, People’s Republic of China 650091 2.Parasitology Department, Kunming Medical College, Kunming, Yunnan, People’s Republic of China 650031 3.Laboratory of Molecular Evolution and Genome Diversity, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, People’s Republic of China 650223 4.Zoology Department, The Natural History Museum, Cromwell Road, London SW7 5BD, U.K. |
| 推荐引用方式 GB/T 7714 | Li QQ,Yang ZQ[*],Zuo YX,et al. A PCR-based RFLP analysis of Sarcocystis cruzi (Protozoa : Sarcocystidae) in Yunnan province, PR china, reveals the water buffalo (Bubalus bubalis) as a natural intermediate host[J]. JOURNAL OF PARASITOLOGY,2002,88(6):1259-1261. |
| APA | Li QQ,Yang ZQ[*],Zuo YX,Attwood SW,Chen XW,&Zhang YP[*].(2002).A PCR-based RFLP analysis of Sarcocystis cruzi (Protozoa : Sarcocystidae) in Yunnan province, PR china, reveals the water buffalo (Bubalus bubalis) as a natural intermediate host.JOURNAL OF PARASITOLOGY,88(6),1259-1261. |
| MLA | Li QQ,et al."A PCR-based RFLP analysis of Sarcocystis cruzi (Protozoa : Sarcocystidae) in Yunnan province, PR china, reveals the water buffalo (Bubalus bubalis) as a natural intermediate host".JOURNAL OF PARASITOLOGY 88.6(2002):1259-1261. |
入库方式： OAI收割
来源：昆明动物研究所
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