Length polymorphism of thymidylate synthase regulatory region in Chinese populations and evolution of the novel alleles
文献类型：期刊论文
| 作者 | Luo HR1; Lu XM1; Yao YG1; Horie N2; Takeishi K2; Jorde LB3; Zhang YP[*]1 |
| 刊名 | BIOCHEMICAL GENETICS
 |
| 出版日期 | 2002 |
| 卷号 | 40期号:1-2页码:41-51 |
| 关键词 | TSER thymidylate synthase length polymorphism Chinese population evolution |
| ISSN号 | 0006-2928 |
| 通讯作者 | zhangyp@public.km.yn.cn |
| 合作状况 | 其它 |
| 英文摘要 | The tandemly repeated 28-bp sequence in the 5'-terminal regulatory, region of human thymidylate synthase (TSER), which has been reported to be polymorphic in different populations, was surveyed in 668 Chinese from 9 Han groups, 8 ethnic populations, and 36 individuals representing a three-generation pedigree. Amplified fragments were separated by electrophoresis on 4% agarose gel. In addition to the reported double and triple repeats of the 28-bp sequence in TSER, we also detected a novel quintuple repeat in this region. The transient expression activity of TSER with the quintuple repeat is almost the same as that of the reported TSER with the triple repeat. All three alleles of the repeat type (2, 3, and 5) were further confirmed by sequencing, The frequencies of the TSER allele 2 and 3 were 18.82 and 81% in totally unrelated Chinese samples, respectively, while the frequency, of allele 3 was variable in different Chinese populations with a range from 62 to 95%. On the basis of the sequences of the different alleles, the existence of the tandem repeats in each allele might be explained by slipped-strand mispairing during DNA replication. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by National Natural Sciences Foundation of China and by NIH Grant No. GM-59290. |
| 原文出处 | 20024041.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/4061]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_重大疾病机理的遗传学 昆明动物研究所_细胞与分子进化开放实验室 |
| 作者单位 | 1.Laboratory of Molecular Evolution and genome diversity, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, People’s Republic of China 2.LaboratoryofGeneticEngineering,SchoolofFoodandNutritionalSciences,UniversityofShizuoka, 52-1 Yada, Shizuoka, Shizuoka 422-8526, Japan 3.Department of Human Genetics, University of Utah Health Sciences Center, Salt Lake City, Utah 84112 |
| 推荐引用方式 GB/T 7714 | Luo HR,Lu XM,Yao YG,et al. Length polymorphism of thymidylate synthase regulatory region in Chinese populations and evolution of the novel alleles[J]. BIOCHEMICAL GENETICS,2002,40(1-2):41-51. |
| APA | Luo HR.,Lu XM.,Yao YG.,Horie N.,Takeishi K.,...&Zhang YP[*].(2002).Length polymorphism of thymidylate synthase regulatory region in Chinese populations and evolution of the novel alleles.BIOCHEMICAL GENETICS,40(1-2),41-51. |
| MLA | Luo HR,et al."Length polymorphism of thymidylate synthase regulatory region in Chinese populations and evolution of the novel alleles".BIOCHEMICAL GENETICS 40.1-2(2002):41-51. |
入库方式： OAI收割
来源：昆明动物研究所
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