Isolation and characterization of microsatellite markers for the endangered Taxus yunnanensis
文献类型：期刊论文
| 作者 | Miao YC1; Su JR[*]1; Zhang ZJ1; Li H2; Luo J3,4; Zhang YP2,3 |
| 刊名 | CONSERVATION GENETICS
 |
| 出版日期 | 2008 |
| 卷号 | 9期号:6页码:1683-1685 |
| 关键词 | SSR-anchored PCR Microsatellite marker SSRs Taxus yunnanensis |
| ISSN号 | 1566-0621 |
| 通讯作者 | jianrongsu@vip.sina.com |
| 合作状况 | 其它 |
| 英文摘要 | Eleven polymorphic microsatellite loci were characterized for the endangered conifer Taxus yunnanensis. Eight loci were isolated through SSR-anchored PCR, one locus was developed by cross-species amplification tests, while the last two loci were obtained from cross-species microsatellite sequences available in GenBank. Variability of these markers was tested in 48 individuals collected from its main distribution range in Southwest China. The number of alleles per locus ranged from 2 to 9, and the observed and expected heterozygosity varied from 0.000 to 0.625 and from 0.062 to 0.853, respectively. Ten of these eleven loci were significantly deviated from Hardy-Weinberg expectation, except TS07 which showed a distinct heterozygote excess. The availability of these new polymorphic microsatellite markers will provide an ideal marker system for detailed population genetics studies in T. yunnanensis and potentially also for closely related species. |
| 收录类别 | SCI |
| 资助信息 | This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) |
| 原文出处 | 200891683.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/4277]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.Research Institute of Resource Insects, Chinese Academy of Forest (CAF), Bailongshi, Kunming 650224, China 2.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 3.Laboratory for Conservation and Utilization of Bio-resource, Yunnan University, Kunming 650091, China 4.School of Life Science, Yunnan University, Kunming 650091, China |
| 推荐引用方式 GB/T 7714 | Miao YC,Su JR[*],Zhang ZJ,et al. Isolation and characterization of microsatellite markers for the endangered Taxus yunnanensis[J]. CONSERVATION GENETICS,2008,9(6):1683-1685. |
| APA | Miao YC,Su JR[*],Zhang ZJ,Li H,Luo J,&Zhang YP.(2008).Isolation and characterization of microsatellite markers for the endangered Taxus yunnanensis.CONSERVATION GENETICS,9(6),1683-1685. |
| MLA | Miao YC,et al."Isolation and characterization of microsatellite markers for the endangered Taxus yunnanensis".CONSERVATION GENETICS 9.6(2008):1683-1685. |
入库方式： OAI收割
来源：昆明动物研究所
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