中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 昆明动物研究所
  3. 昆明动物研究所
  4. 遗传资源与进化国家重点实验室
  5. 分子进化基因组学
Non-invasive methods for identifying oocysts of Sarcocystis spp. from definitive hosts

文献类型：期刊论文

作者Xiang Z1,6; Chen XW2; Yang LJ1; He YS1; Jiang RS3; Rosenthal BM4; Luan PT6; Attwood SW5; Zuo YX2; Zhang YP[*]6,7
刊名PARASITOLOGY INTERNATIONAL
出版日期2009
卷号58期号:3页码:293-296
关键词Sarcocystis Oocysts Faeces Taxonomy 18S rRNA Diagnostics
ISSN号1383-5769
通讯作者zhaoqingy@yahoo.com
合作状况其它
英文摘要Because the excreted sporocysts and/or oocysts of various species of Sarcocystis may not be discriminated morphologically, we sought to validate a diagnostic technique based on variation in the 18S rDNA sequence. Oocysts and/or sporocysts; from three taxa of Sarcocystis were collected from human, feline, and canine definitive hosts that had fed upon meats infected with the muscle cysts of Sarcocystis hominis, Sarcocystis fusiformis and a species of Sarcocystis from water buffalo that could not be distinguished from Sarcocystis cruzi. Using a new collection method employing filter paper, these excreted oocysts and sporocysts were subjected to DNA extraction, as were the corresponding muscle cysts. Methods employing PCR-RFLP and DNA sequencing of a partial 18S rDNA gene (ssrRNA) sequence were then used to successfully distinguish among the three taxa. The same, unique restriction digestion pattern characterizes the tissue cysts and oocysts and/or sporocysts of each parasite taxon. The technique makes possible amplification and identification of species specific gene sequences based on DNA extracted from as few as 7 excreted sporocysts (the equivalent of 3 and 1/2 oocysts) from freshly prepared material, or as few as 50 sporocysts from feces samples that had been stored in potassium dichromate (K(2)Cr(2)O(7)) for as long as 6 years. This represents the first report using molecular diagnostic procedures to diagnose oocysts of Sarcocystis in faecal samples, describing a valuable new tool for studying the epidemiology of various Sarcocystis species.
学科主题Parasitology
收录类别SCI
资助信息This study was funded by The National Natural Science Foundation of China (nos. 30560022, 30621092), and The Foundation of Kunming Medical College for Science and Advanced Academic Teacher. Dr S W Attwood was supported by Wellcome Trust Project Grant (no.
原文出处2009583293.pdf
语种英语
公开日期2010-08-24
源URL[http://159.226.149.42:8088/handle/152453/4317]  
专题昆明动物研究所_分子进化基因组学
昆明动物研究所_遗传资源与进化国家重点实验室
作者单位1.Parasitology Department, Kunming Medical College, Kunming, Yunnan 650031, PR China
2.Department of Biology, Yunnan University, Kunming, Yunnan 650091, PR China
3.Division of Health Social Sciences, Institute for Health Science, Kunming Medical College, Kunming, Yunnan 650031, PR China
4.Animal Parasitic Disease Laboratory, Agricultural Research Service, US Department of Agriculture, BARC East Building, 1180 Beltsville, MD 20705, USA
5.State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, PR China
6.Laboratory for Conservation and Utilization of Bioresource, Yunnan University, Kunming 650091, PR China
7.State Key Laboratory of Genetic Resource and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, PR China
推荐引用方式
GB/T 7714		 Xiang Z,Chen XW,Yang LJ,et al. Non-invasive methods for identifying oocysts of Sarcocystis spp. from definitive hosts[J]. PARASITOLOGY INTERNATIONAL,2009,58(3):293-296.
APA Xiang Z.,Chen XW.,Yang LJ.,He YS.,Jiang RS.,...&Yang ZQ[*].(2009).Non-invasive methods for identifying oocysts of Sarcocystis spp. from definitive hosts.PARASITOLOGY INTERNATIONAL,58(3),293-296.
MLA Xiang Z,et al."Non-invasive methods for identifying oocysts of Sarcocystis spp. from definitive hosts".PARASITOLOGY INTERNATIONAL 58.3(2009):293-296.

入库方式： OAI收割

来源：昆明动物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。