Universal primers for amplifying the complete coding sequence of cytoplasmic heat shock protein 90 (HSP90) in Lepidoptera
文献类型：期刊论文
| 作者 | Xu PJ1,4; Li T1,4; Xiao JH1; Murphy RW3,5; Huang DW[*]1,2 |
| 刊名 | EUROPEAN JOURNAL OF ENTOMOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 108期号:1页码:164-168 |
| 关键词 | coding sequence untranslated region HSP90 RACE Universal primer Lepidoptera |
| 通讯作者 | huangdw@ioz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Using sequence alignment, a conserved domain in the 3' untranslated region (UTR) of the cytoplasmic heat shock protein 90 (HSP90) of Lepidoptera was found. This region is highly variable in other insect groups. Furthermore, universal primers were designed to amplify the complete coding sequence (CDS) of HSP90 from total genomic DNA in Lepidoptera, avoiding the commonly used reverse transcription-polymerase chain reaction (RT-PCR) and 3', 5'-rapid amplification of cDNA ends (RACE) methods based on cDNA. These primers amplified a fragment of about 2.25 kb in the 11 species tested, which represent seven different families of Lepidoptera, including moths and butterflies. The results suggest that the conserved domain of 3' UTR is universal in Lepidoptera and these primers successfully amplify the complete CDS of cytoplasmic HSP90 from genomic DNA. |
| 收录类别 | SCI |
| 资助信息 | This project was supported by the National Natural Science Foundation of China (NSFC grant no. 30770302, 30570970), partially by Program of Ministry of Sci- ence and Technology of the Republic of China (2006FY110500) and by National Science Fund for Fostering Talents in Basic Research (Special subjects in animal taxonomy, NSFC- J0630964/J0109). |
| 语种 | 英语 |
| 公开日期 | 2011-03-16 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6585]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing 100101, China 2.College of Life Sciences, Hebei University, Baoding, 071002, China 3.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 4.Graduate School of the Chinese Academy of Sciences, Beijing, China 5.Department of Natural History, Royal Ontario Museum, 100 Queen’s Park, Toronto, Ontario M5S 2C6, Canada |
| 推荐引用方式 GB/T 7714 | Xu PJ,Li T,Xiao JH,et al. Universal primers for amplifying the complete coding sequence of cytoplasmic heat shock protein 90 (HSP90) in Lepidoptera[J]. EUROPEAN JOURNAL OF ENTOMOLOGY,2011,108(1):164-168. |
| APA | Xu PJ,Li T,Xiao JH,Murphy RW,&Huang DW[*].(2011).Universal primers for amplifying the complete coding sequence of cytoplasmic heat shock protein 90 (HSP90) in Lepidoptera.EUROPEAN JOURNAL OF ENTOMOLOGY,108(1),164-168. |
| MLA | Xu PJ,et al."Universal primers for amplifying the complete coding sequence of cytoplasmic heat shock protein 90 (HSP90) in Lepidoptera".EUROPEAN JOURNAL OF ENTOMOLOGY 108.1(2011):164-168. |
入库方式： OAI收割
来源：昆明动物研究所
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