Identification of two metallothionein genes and their roles in stress responses of Musca domestica toward hyperthermy and cadmium tolerance
文献类型：期刊论文
| 作者 | Tang T1; Huang DW1; Zhang D1; Wu YJ1; Murphy RW2,3; Liu FS[*]1 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 160期号:2-3页码:81-88 |
| 关键词 | Musca domestica Metallothionein Cadmium Heat shock Stress response |
| 通讯作者 | liufengsong@hbu.edu.cn |
| 合作状况 | 其它 |
| 英文摘要 | Stress proteins such as metallothioneins (MTs) play a key role in cellular protection against environmental stressors. In nature, insects such as houseflies (Musca domestica) are commonly exposed to multiple stressors including heavy metals (e.g. Cadmium, Cd) and high temperatures. In this paper, we identify two novel MT genes from the cDNAs of M. domestica, MdMT1 and MdMT2, which putatively encode 40 and 42 amino acid residues respectively. Expression of the two MTs' mRNAs, which are examined in the fat body, gut, hemocyte, and the epidermis. From our study, we saw that the expression of MdMT1 and MdMT2 are enhanced by Cd and thermal stress. Levels of expression are highest at 10 mM Cd(2+) within a 24-h period, and expressions increase significantly with exposure to 10 mM Cd for 12 h. Levels of the mRNAs are up-regulated after heat shock and that of MdMT2 reaches its maximum peak faster than MdMT1. Both of the MT genes might be involved in a transient systemic tolerance response to stressors and they may play important roles in heavy metal and high temperature tolerance in the housefly. To detect whether or not the MTs bind heavy metals, the target genes are cloned into the prokaryotic expression vector pET-DsbA to obtain fusion protein expressed in Escherichia coli BL21 (DE3). Recombinant DsbA-MdMT1 significantly increases tolerance of the host bacteria to Cd(2+), but DsbA-MdMT2 is absent These differential characteristics will facilitate future investigations into the physiological functions of MTs. |
| 收录类别 | SCI |
| 资助信息 | This work wasfinancially supported by the Projectsupportedbythe SpecializedResearchFundfortheDoctoralProgramofHigherEducation of China (No. 20101301120005), Natural Science Foundation of Hebei University (No. 2010001), and Natural Science Foundation of Hebei Province (No. C2011201027). |
| 语种 | 英语 |
| 公开日期 | 2011-11-08 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6822]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.College of Life Sciences, Hebei University, Baoding, Hebei, 071002, China 2.Centre for Biodiversity and Conservation Biology, Royal Ontario Museum, 100 Queen's Park, Toronto, Ontario, Canada M5S 2C6 3.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan, 650223, China |
| 推荐引用方式 GB/T 7714 | Tang T,Huang DW,Zhang D,et al. Identification of two metallothionein genes and their roles in stress responses of Musca domestica toward hyperthermy and cadmium tolerance[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2011,160(2-3):81-88. |
| APA | Tang T,Huang DW,Zhang D,Wu YJ,Murphy RW,&Liu FS[*].(2011).Identification of two metallothionein genes and their roles in stress responses of Musca domestica toward hyperthermy and cadmium tolerance.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,160(2-3),81-88. |
| MLA | Tang T,et al."Identification of two metallothionein genes and their roles in stress responses of Musca domestica toward hyperthermy and cadmium tolerance".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 160.2-3(2011):81-88. |
入库方式： OAI收割
来源：昆明动物研究所
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