热门
Universal COI primers for DNA barcoding amphibians
文献类型：期刊论文
| 作者 | Che J1; Chen HM1,2; Yang JX1; Jin JQ1; Jiang K1; Yuan ZY1; Murphy RW[*]1,3; Zhang YP[*]1,2 |
| 刊名 | MOLECULAR ECOLOGY RESOURCES
 |
| 出版日期 | 2012 |
| 卷号 | 12期号:2页码:247-258 |
| 关键词 | Anura Caudata forensics genomics Gymnophiona |
| 通讯作者 | bob.murphy@utoronto.ca ; zhangyp@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | DNA barcoding is a proven tool for the rapid and unambiguous identification of species, which is essential for many activities including the vouchering tissue samples in the genome 10K initiative, genealogical reconstructions, forensics and biodiversity surveys, among many other applications. A large-scale effort is underway to barcode all amphibian species using the universally sequenced DNA region, a partial fragment of mitochondrial cytochrome oxidase subunit I COI. This fragment is desirable because it appears to be superior to 16S for barcoding, at least for some groups of salamanders. The barcoding of amphibians is essential in part because many species are now endangered. Unfortunately, existing primers for COI often fail to achieve this goal. Herein, we report two new pairs of primers (?, ?) that in combination serve to universally amplify and sequence all three orders of Chinese amphibians as represented by 36 genera. This taxonomic diversity, which includes caecilians, salamanders and frogs, suggests that the new primer pairs will universally amplify COI for the vast majority species of amphibians |
| 资助信息 | This work was supported by grants from the Ministry of Science and Technology of China (MOST Grant 2011FY120200), the National Natural Science Foundation of China (31090100), Chi- nese Academy of Sciences (KSCX2-YW-Z-0807, KSCX2-EW-Z-2), and the Bureau of Science and Technology of Yunnan Province to Y.P.Z and J.C. This work was supported in part by a Visiting Professorship for Senior International Scientists from the Chi- nese Academy of Sciences to R.W.M. Manuscript preparation was supported by a Natural Sciences and Engineering Research Council of Canada Discovery Grant A3148. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000299930300007 |
| 公开日期 | 2012-03-19 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6876]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_两栖爬行类多样性与进化 |
| 作者单位 | 1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091, China 3.Centre for Biodiversity and Conservation Biology, Royal Ontario Museum, 100 Queen’s Park, Toronto, Ontario M5S 2C6, Canada |
| 推荐引用方式 GB/T 7714 | Che J,Chen HM,Yang JX,et al. Universal COI primers for DNA barcoding amphibians[J]. MOLECULAR ECOLOGY RESOURCES,2012,12(2):247-258. |
| APA | Che J.,Chen HM.,Yang JX.,Jin JQ.,Jiang K.,...&Zhang YP[*].(2012).Universal COI primers for DNA barcoding amphibians.MOLECULAR ECOLOGY RESOURCES,12(2),247-258. |
| MLA | Che J,et al."Universal COI primers for DNA barcoding amphibians".MOLECULAR ECOLOGY RESOURCES 12.2(2012):247-258. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。