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COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae)
文献类型：期刊论文
| 作者 | Xia Y1,2; Gu HF1,2; Peng R1; Chen Q1; Zheng YC1; Murphy RW3,4; Zeng XM[*]1 |
| 刊名 | MOLECULAR ECOLOGY RESOURCES
 |
| 出版日期 | 2012 |
| 卷号 | 12期号:1页码:48-56 |
| 关键词 | China cryptic species Japan South Korea species identification |
| 通讯作者 | zengxm@cib.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | The 5' region of the mitochondrial DNA (mtDNA) gene cytochrome c oxidase I (COI) is the standard marker for DNA barcoding. However, because COI tends to be highly variable in amphibians, sequencing is often challenging. Consequently, another mtDNA gene, 16S rRNA gene, is often advocated for amphibian barcoding. Herein, we directly compare the usefulness of COI and 16S in discriminating species of hynobiid salamanders using 130 individuals. Species identification and classification of these animals, which are endemic to Asia, are often based on morphology only. Analysis of Kimura 2-parameter genetic distances (K2P) documents the mean intraspecific variation for COI and 16S rRNA genes to be 1.4% and 0.3%, respectively. Whereas COI can always identify species, sometimes 16S cannot. Intra- and interspecific genetic divergences occasionally overlap in both markers, thus reducing the value of a barcoding gap to identify genera. Regardless, COI is the better DNA barcoding marker for hynobiids. In addition to the comparison of two potential markers, high levels of intraspecific divergence in COI (>5%) suggest that both Onychodactylus fischeri and Salamandrella keyserlingii might be composites of cryptic species. |
| 收录类别 | SCI |
| 资助信息 | This research was supported by the National Natural Sciences Foundation of China (NSFC-30870287 and NSFC-30900134) and by the Chinese Academy of Sciences (09C3011100, KSCX2-EW-J-22 and KSCX2-YW-Z-0906). Manu- script preparation was supported, in part, by the Natural Sciences and Engineering Research Council of Canada (A3148). |
| 语种 | 英语 |
| WOS记录号 | WOS:000298585000005 |
| 公开日期 | 2012-03-20 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6895]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2.Graduate University of Chinese Academy of Sciences, Beijing, 100049, China 3.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 4.Centre for Biodiversity and Conservation Biology, Royal Ontario Museum, 100 Queen’s Park, Toronto, Ontario M5S 2C6, Canada |
| 推荐引用方式 GB/T 7714 | Xia Y,Gu HF,Peng R,et al. COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae)[J]. MOLECULAR ECOLOGY RESOURCES,2012,12(1):48-56. |
| APA | Xia Y.,Gu HF.,Peng R.,Chen Q.,Zheng YC.,...&Zeng XM[*].(2012).COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae).MOLECULAR ECOLOGY RESOURCES,12(1),48-56. |
| MLA | Xia Y,et al."COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae)".MOLECULAR ECOLOGY RESOURCES 12.1(2012):48-56. |
入库方式： OAI收割
来源：昆明动物研究所
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