Tissue sampling methods and standards for vertebrate genomics
文献类型：期刊论文
| 作者 | Wong PBY1,2; Wiley EO3; Johnson WE4; Ryder OA5; O'Brien SJ6; Haussler D7; Koepfli KP4; Houck ML5; Perelman P8; Mastromonaco G9 |
| 刊名 | GIGASCIENCE
 |
| 出版日期 | 2012 |
| 卷号 | 1期号:X页码:e8 |
| 关键词 | Genome 10K Sequencing Vertebrates Genomics Tissue sampling Tissue storage Cell line Tissue culture RNA DNA |
| 通讯作者 | bob.murphy@utoronto.ca |
| 合作状况 | 其它 |
| 英文摘要 | The recent rise in speed and efficiency of new sequencing technologies have facilitated high-throughput sequencing, assembly and analyses of genomes, advancing ongoing efforts to analyze genetic sequences across major vertebrate groups. Standardized procedures in acquiring high quality DNA and RNA and establishing cell lines from target species will facilitate these initiatives. We provide a legal and methodological guide according to four standards of acquiring and storing tissue for the Genome 10K Project and similar initiatives as follows: four-star (banked tissue/cell cultures, RNA from multiple types of tissue for transcriptomes, and sufficient flash-frozen tissue for 1 mg of DNA, all from a single individual); three-star (RNA as above and frozen tissue for 1 mg of DNA); two-star (frozen tissue for at least 700 mug of DNA); and one-star (ethanol-preserved tissue for 700 mug of DNA or less of mixed quality). At a minimum, all tissues collected for the Genome 10K and other genomic projects should consider each species' natural history and follow institutional and legal requirements. Associated documentation should detail as much information as possible about provenance to ensure representative sampling and subsequent sequencing. Hopefully, the procedures outlined here will not only encourage success in the Genome 10K Project but also inspire the adaptation of standards by other genomic projects, including those involving other biota. |
| 资助信息 | This research was supported by a Natural Sciences and Research Council of Canada Discovery Grant 3148 and by a Visiting Professorship for Senior International Scientists from the Chinese Academy of Sciences to RWM. This effort was also supported by the National Science Foundation Assembling the Tree of Life Grant DEB0732819 to EOW. |
| 收录类别 | 其他 |
| 语种 | 英语 |
| 公开日期 | 2013-09-16 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7638]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks St., Toronto, Ontario M5S 3B2, Canada 2.Centre for Biodiversity and Conservation Biology, Royal Ontario Museum, 100 Queen`s Park, Toronto, Ontario M5S 2C6, Canada 3.Division of Ichthyology, Biodiversity Institute, University of Kansas, 1345 Jayhawk Boulevard, Lawrence, Kansas 66045, USA 4.Laboratory of Genomic Diversity, National Cancer Institute, 31 Center Drive, Frederick, Maryland 21702-1201, USA 5.San Diego Zoo Institute for Conservation Research, 15600 San Pasqual Valley Road, Escondido, California 92027, USA 6.Theodosius Dobzhansky Center for Genome Bioinformatics, St. Petersburg State University, 8 Viborgskaya Street, St. Petersburg 194044, Russia 7.Center for Biomolecular Science and Engineering, University of California Santa Cruz, 1156 High Street, Santa Cruz, California 95064, USA. 8.Laboratory of Animal Cytogenetics, Institute of Molecular and Cellular Biology, Lavrentiev 10, Novosibirsk 630090, Russia 9.Reproductive Physiology, Toronto Zoo, 361A Old Finch Avenue, Scarborough, Ontario M1B 5K7, Canada 10.ASTAR, Inst Mol & Cell Biol, Comparat Genom Lab, Biopolis, Singapore 138673, Singapore |
| 推荐引用方式 GB/T 7714 | Wong PBY,Wiley EO,Johnson WE,et al. Tissue sampling methods and standards for vertebrate genomics[J]. GIGASCIENCE,2012,1(X):e8. |
| APA | Wong PBY.,Wiley EO.,Johnson WE.,Ryder OA.,O'Brien SJ.,...&Murphy RW[*].(2012).Tissue sampling methods and standards for vertebrate genomics.GIGASCIENCE,1(X),e8. |
| MLA | Wong PBY,et al."Tissue sampling methods and standards for vertebrate genomics".GIGASCIENCE 1.X(2012):e8. |
入库方式： OAI收割
来源：昆明动物研究所
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