A genome draft of the legless anguid lizard, Ophisaurus gracilis
文献类型：期刊论文
| 作者 | Song B1; Cheng SF1,2; Sun YB3; Zhong X1; Jin JQ3; Guan R1; Murphy RW3,4; Che J3; Zhang YP3,5; Liu X[*]1 |
| 刊名 | GIGASCIENCE
 |
| 出版日期 | 2015 |
| 卷号 | 4期号:X页码:e17 |
| 关键词 | Anguidae Limblessness Lizard genome Squamate reptiles |
| 通讯作者 | liuxin@genomics.cn |
| 英文摘要 | BACKGROUND: Transition from a lizard-like to a snake-like body form is one of the most important transformations in reptilian evolution. The increasing number of sequenced reptilian genomes is enabling a deeper understanding of vertebrate evolution, although the genetic basis of the loss of limbs in reptiles remains enigmatic. Here we report genome sequencing, assembly, and annotation for the Asian glass lizard Ophisaurus gracilis, a limbless lizard species with an elongated snake-like body form. Addition of this species to the genome repository will provide an excellent resource for studying the genetic basis of limb loss and trunk elongation. FINDINGS: O. gracilis genome sequencing using the Illumina HiSeq2000 platform resulted in 274.20 Gbp of raw data that was filtered and assembled to a final size of 1.78 Gbp, comprising 6,717 scaffolds with N50=1.27 Mbp. Based on the k-mer estimated genome size of 1.71 Gbp, the assembly appears to be nearly 100% complete. A total of 19,513 protein-coding genes were predicted, and 884.06 Mbp of repeat sequences (approximately half of the genome) were annotated. The draft genome of O. gracilis has similar characteristics to both lizard and snake genomes. CONCLUSIONS: We report the first genome of a lizard from the family Anguidae, O. gracilis. This supplements currently available genetic and genomic resources for amniote vertebrates, representing a major increase in comparative genome data available for squamate reptiles in particular. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from the Strategic Priority Research Program (B) (XDB13020200). |
| 语种 | 英语 |
| 公开日期 | 2015-06-09 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8351]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_两栖爬行类多样性与进化 |
| 作者单位 | 1.BGI-Shenzhen, Shenzhen 518083, China 2.HKU-BGI Bioinformatics Algorithms and Core Technology Research Laboratory, The Computer Science Department, The University of Hong Kong, Hong Kong, China 3.State Key Laboratory of Genetic Resources and Evolution, and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 4.Centre for Biodiversity and Conservation Biology, Royal Ontario Museum, 100 Queen’s Park, Toronto, Ont. M5S 2C6, Canada 5.Laboratory for Conservation and Utilization of Bio-resource, Yunnan University, Kunming 650091, China |
| 推荐引用方式 GB/T 7714 | Song B,Cheng SF,Sun YB,et al. A genome draft of the legless anguid lizard, Ophisaurus gracilis[J]. GIGASCIENCE,2015,4(X):e17. |
| APA | Song B.,Cheng SF.,Sun YB.,Zhong X.,Jin JQ.,...&Liu X[*].(2015).A genome draft of the legless anguid lizard, Ophisaurus gracilis.GIGASCIENCE,4(X),e17. |
| MLA | Song B,et al."A genome draft of the legless anguid lizard, Ophisaurus gracilis".GIGASCIENCE 4.X(2015):e17. |
入库方式： OAI收割
来源：昆明动物研究所
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