Proteomic analysis of the skin of Chinese giant salamander (Andrias dauidianus)
文献类型：期刊论文
| 作者 | Geng XF1,2,5; Wei H3; Shang HT4; Zhou MH4; Chen B4; Zhang FC5; Zang XY1,2; Li PF1,2; Sun JY1,2; Che J6,7 |
| 刊名 | JOURNAL OF PROTEOMICS
 |
| 出版日期 | 2015 |
| 卷号 | 119期号:X页码:196-208 |
| 关键词 | Chinese giant salamander Proteome Skin Mucus Molting Defense |
| 通讯作者 | cellkeylab@126.com |
| 合作状况 | 其它 |
| 英文摘要 | The Chinese giant salamander (Andrias davidianus), renowned as a living fossil, is the largest and longest-lived amphibian species in the world. Its skin has developed mucous gland which could secrete a large amount of mucus under the scraping and electric stimulation, and the molting is the degraded skin stratum comeum. Although several proteomic studies have focused on functional proteomes of mammalian and frog skin, the skin proteome of Chinese giant salamander has not yet been carefully studied. To establish the functional skin proteome of Chinese giant salamander, two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS) were applied to detect the composition and relative abundance of the proteins in the skin, mucus and molting. Our findings indicated that 249 proteins were identified in the skin, 155 proteins in the mucus, and 97 proteins in the molting. Furthermore, Gene Ontology (GO) analysis showed that these proteins participated in various physiological activities, including extracellular matrix organization, defense, immune response, wound healing, respiration, etc. In conclusion, the proteomic results provide new insight in the aspects of the proteomes in the skin, mucus and the molting of Chinese giant salamander. Biological significance This was the first study to examine the protein expression abundance in the skin, mucus and molting of Chinese giant salamander by a proteomics approach. Meantime, the identification of a more global proteome in normal skin may provide a basis for characterizing and comparing the skin proteomes from other amphibian species. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from the National Basic Research 973 Pre-research Program of China (No. 2012CB722304), the Major Scientific and Technological Projects of Henan (111100910600), and the Foundation and Advanced Tech- nology Research Program of Henan (No. 132300413208, No. 132300410134 and No. 142300413212). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9330]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_两栖爬行类多样性与进化 |
| 作者单位 | 1.State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang 453007, China 2.Henan Bioengineering Key Laboratory, Henan Normal University, Xinxiang 453007, China 3.Department of Laboratory Animal Science, Third Military Medical University, Chongqing 400038, China 4.Chongqing Kui Xu Biotechnology Incorporated Company, Kaixian Country, Chongqing 405423, China 5.Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China 6.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 7.Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China |
| 推荐引用方式 GB/T 7714 | Geng XF,Wei H,Shang HT,et al. Proteomic analysis of the skin of Chinese giant salamander (Andrias dauidianus)[J]. JOURNAL OF PROTEOMICS,2015,119(X):196-208. |
| APA | Geng XF.,Wei H.,Shang HT.,Zhou MH.,Chen B.,...&Xu CS[*].(2015).Proteomic analysis of the skin of Chinese giant salamander (Andrias dauidianus).JOURNAL OF PROTEOMICS,119(X),196-208. |
| MLA | Geng XF,et al."Proteomic analysis of the skin of Chinese giant salamander (Andrias dauidianus)".JOURNAL OF PROTEOMICS 119.X(2015):196-208. |
入库方式： OAI收割
来源：昆明动物研究所
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