Expression of BSE-associated proteins in the CNS and lymphoreticular tissues of cattle and buffalo
文献类型：期刊论文
| 作者 | Zhao H1; Wang SQ1,2; Qing LL1,2; Liu LL1,2; Zhang YP[*]1,3 |
| 刊名 | SCIENCE BULLETIN
 |
| 出版日期 | 2016 |
| 卷号 | 61期号:17页码:1377-1383 |
| 关键词 | BSE PRNP SPRN Cattle Buffalo |
| 通讯作者 | zhangyp@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Bovine spongiform encephalopathy (BSE), which has been documented in 190,000 cases of BSE-infected cattle, to our knowledge, has not been reported in buffalo. Prion protein (PrP) is critical to susceptibility and development of BSE. Moreover, a new PrP-like protein, Shadoo (Sho), has been shown to have overlapping expression patterns and shared functions with PrP. Therefore, we hypothesize that differences in expression at the transcriptional level and/or the post-transcriptional level of the two genes may be associated with these specific differences between cattle and buffalo. We compared the relative mRNA expression of the prion protein gene (PRNP) and the Shadoo gene (SPRN) in 396 RNA samples using real-time PCR. We also analyzed PrP/Sho protein from 96 samples by Western blot. Our results demonstrated significantly lower PrP expression in the cerebellum, obex, mesenteric lymph node, and bronchial lymph node tissues, but higher relative expression of Sho in the cerebrum and spleen in buffalos compared with cattles. Although these results support our primary assumption, Sho and PrP expressions did not correlate with corresponding mRNA expression, suggesting that the biological modulations of both PrP and Sho proteins are at post-translational levels. Moreover, positive correlations between PRNP and SPRN were found in cattle and buffalo cerebrum as well as in buffalo obex. Conversely, negative correlations between PrP and Sho were detected in buffalo cerebellum and obex tissues. These findings suggest that additional post-transcriptional studies are warranted to elucidate mechanisms behind prion diseases. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the National Natural Science Foundation of China (31060302, 31260032 and 31460038), and the Program for Innovative Research Team (in Science and Technology) in the University of Yunnan Province. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10230]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.State Key Laboratory for Conservation and Utilization of Bio- resource, Yunnan University, Kunming 650091, China 2.School of Life Science, Yunnan University, Kunming 650091, China 3.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China |
| 推荐引用方式 GB/T 7714 | Zhao H,Wang SQ,Qing LL,et al. Expression of BSE-associated proteins in the CNS and lymphoreticular tissues of cattle and buffalo[J]. SCIENCE BULLETIN,2016,61(17):1377-1383. |
| APA | Zhao H,Wang SQ,Qing LL,Liu LL,&Zhang YP[*].(2016).Expression of BSE-associated proteins in the CNS and lymphoreticular tissues of cattle and buffalo.SCIENCE BULLETIN,61(17),1377-1383. |
| MLA | Zhao H,et al."Expression of BSE-associated proteins in the CNS and lymphoreticular tissues of cattle and buffalo".SCIENCE BULLETIN 61.17(2016):1377-1383. |
入库方式： OAI收割
来源：昆明动物研究所
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